Thermo Scientific EZ-Link PFP-biotin is a pentafluorophenyl-ester biotinylation reagent that has similar properties to NHS-biotin but is more reactive and will target both primary and secondary amines for biotinylation.
PFP-Biotin is an amine-reactive biotinylation reagent based on activation as as a pentafluorophenyl ester (PFP ester) rather than the usual N-hydroxysuccinimide (NHS) ester. The compound has properties similar to those of NHS-Biotin (Part No. 20217) but exhibits greater reactivity toward amino groups. Unlike NHS-Biotin, PFP-Biotin will react with both secondary amines and primary amines, enabling it to be used to label nucleic acids as well as proteins. The reaction occurs spontaneously at pH 7 to 9.
- Molecular labeling – biotinylate proteins and nucleic acids for avidin-biotin methods
- Membrane-permeable – can be used to label inside cells (intracellular)
- Amine-reactive – reacts with primary and secondary amines of proteins and other molecules (unlike typical NHS reagents, which react with primary amines only)
- Irreversible – forms permanent amide bonds; spacer arm cannot be cleaved
- Solubility – must be dissolved in DMSO or DMF before further dilution in aqueous buffers
- Very short – spacer arm (total length added to target) is 9.6 angstroms; it consists of the native biotin valeric acid group only
||Chemical structure of PFP-Biotin (biotin pentafluorophenyl ester). This biotinylation reagent labels proteins, nucleic acids and other molecules that contain primary amines or secondary amines. For more information, see our review of Amine-reactive Crosslinker Chemistry.
Properties of PFP-Biotin.
||Biotin PFP ester
|Spacer arm length
||White to off-white solid
||25mg/mL in DMF
||4°C with desiccant, protect from moisture, use only fresh solutions
||Pentafluorophenyl ester, reacts with primary amines at pH 7.0-9.0
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
- Muroi, M., et al. (2002). J. Biol. Chem. 277, 42372-42379.
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
All Amine-Reactive Biotinylation Reagents
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins