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EZ-Link Maleimide-PEG2-Biotin2


A simple, water-soluble biotinylation reagent specific for sulfhydryls.

EZ-Link Maleimide-PEG2-Biotin

Thermo Scientific EZ-Link Maleimide-PEG2-Biotin is a mid-length, maleimide-activated, sulfhydryl-reactive biotinylation reagent that contains a 2-unit ethylene glycol in its spacer arm for increased water-solubility characteristics.

Maleimide-PEG2-Biotin enables simple and efficient biotinylation of antibodies, cysteine-containing peptides and other thiol-containing molecules. The maleimide group reacts specifically and efficiently with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds. The hydrophilic, 2-unit polyethylene glycol (PEG) spacer arm imparts water solubility that is transferred to the biotinylated molecule, thus reducing aggregation of labeled proteins stored in solution. The PEG segment adds length and flexibility to the spacer arm, minimizing steric hindrance involved with binding to avidin molecules.

Highlights:

  • Protein labeling – biotinylate antibodies or other proteins for use in protein methods
  • Thiol-reactive – reacts with sulfhydryls (-SH), such as the side-chain of cysteine (C)
  • Maleimide-activated – perform reactions at pH 6.5 to 7.5 in buffers such as PBS
  • Pegylated – spacer arm contains a hydrophilic, 2-unit, polyethylene glycol (PEG) group
  • Enhances solubility – pegylation imparts water solubility to the biotinylated molecule, helping to prevent aggregation of biotinylated antibodies stored in solution
  • Irreversible – forms permanent thioether bonds; spacer arm cannot be cleaved
  • Solubility – can be dissolved directly in aqueous buffers for labeling reactions
  • Medium length – spacer arm (total length added to target) is 29.1 angstroms

Product Details:

Chemical structure of Maleimide-PEG2-Biotin
Chemical structure of Maleimide-PEG2-Biotin. This biotinylation reagent labels antibodies and other proteins and molecules that contain sulfhydryl groups (e.g., side-chain of cysteine). For more information, see our review of Maleimide Reaction Chemistry.

Properties of Maleimide-PEG-Biotin Labeling Reagents. These sulfhydryl-reactive biotinylation compounds have hydrophilic, water-soluble spacer arms composed of 2 or 11 ethylene glycol groups.
  Maleimide-PEG2-Biotin Maleimide-PEG11-Biotin
Alternative names Maleimide-PEO2-Biotin
Mal-dPEG2-Biotin
Maleimido-PEG2-Biotin
Maleimide-PEO11-Biotin
Mal-dPEG11-Biotin
Maleimido-PEG11-Biotin
Molecular formula C23H35N5O7S C41H71N5O16S
Molecular weight 525.62 922.09
Spacer arm length 29.1 angstroms 59.1 angstroms
Mass added to target 525.23 daltons 921.70 daltons
Form White to off-white solid White to off-white solid
Solubility at least 25mg/mL in water or buffer

best to make DMF or DMSO stock

Storage conditions 4°C with desiccant
Reactive groups Maleimide, reacts with sulfhydryls at pH 6.5-7.5
SMILES structure of Maleimide-PEG2-Biotin:
O=C1NC2C(C(CCCCC(NCCOCCOCCNC(CCN3C(C=CC3=O)=O)=O)=O)SC2)N1

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Biotinylation reagents differ in reactivity, length, solubility, cell permeability and cleavability. Three types of sulfhydryl-reactive compounds are available: maleimido, iodoacetyl and pyridyldithiol. Maleimide reagents specifically react with sulfhydryl groups (-SH) in near-neutral buffers to form permanent thioether bonds.

In proteins, sulfhydryls exist where there are cysteine (C) residues. Cystine disulfide bonds must be reduced to make sulfhydryl groups available for labeling. Hinge-region disulfide bridges of antibodies can be selectively reduced to make functional half-antibodies that can be labeled.

References:

  1. Oda, Y, et al. (2001) Nature Biotechnology 19, 379-382.
  2. Khurana, S., et al. (2005) J. Virol. 79, 6791-6800.
  3. Golding, H., et al. (2005) J. Biol. Chem. 280, 29570-29577.

Related Resources:

Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)

Related Products:

Maleimide Solid Phase Biotinylation Kit
All Biotinylation Reagents
Biotin Quantitation Reagents and Kits
Avidin, Streptavidin, NeutrAvidin Affinity Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21901 EZ-Link Maleimide PEG2-Biotin
Formulation: Maleimide-activated biotin with diethyleneglycol spacer
Sufficient For: Approx. 25 uses to label a total of 634mg of IgG using typical conditions
50mg Product Instructions for product #21901 EZ-Link Maleimide PEG2-Biotin MSDS for product #21901 EZ-Link Maleimide PEG2-Biotin Certificate of Analysis for product #21901 EZ-Link Maleimide PEG2-Biotin $207.00

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21902 EZ-Link Maleimide-PEG2-Biotin, No-Weigh Format
Formulation: Maleimide-activated biotin with diethyleneglycol spacer
Sufficient For: 8 uses to label a total of 203mg of IgG using typical conditions
8 x 2mg Product Instructions for product #21902 EZ-Link Maleimide-PEG2-Biotin, No-Weigh Format MSDS for product #21902 EZ-Link Maleimide-PEG2-Biotin, No-Weigh Format Certificate of Analysis for product #21902 EZ-Link Maleimide-PEG2-Biotin, No-Weigh Format $201.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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