Thermo Scientific EZ-Link Amine-PEG11-Biotin is a biotinylation reagent containing a long 11-unit polyethylene glycol (PEG) spacer arm and a terminal primary amine group for conjugation and protein labeling with EDC or other crosslinkers.
Amine-PEG11-Biotin is the longest of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The 11-unit PEG segment is hydrophilic and confers greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amine of this pegylated biotin reagent can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the –NH2 group of the amino-biotin, forming an amide bond.
- Biotinylation – label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
- Amine-activated – primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
- Pegylated – the 11-unit, polyethylene glycol (PEG) group in spacer arm enhances water solubility of biotinylated molecules
- Very long reach – total reagent length is more than 50 angstroms, greatly minimizing steric hindrance for the biotin binding-function
|Chemical structure of Amine-PEG11-Biotin. Amine-terminated biotinylation reagents such as this one are often conjugated to carboxyl groups using the crosslinker EDC. For more information, see our review of Carbodiimide Reaction Chemistry.
Properties of Amine-PEG11-Biotin. Also see Amine-PEG2-Biotin.
|Spacer arm length
|Mass added to target
||752.43 daltons (via EDC to a carboxyl group)
||White to off-white solid
||At least 25mg/mL in water
||-20°C; shipped at ambient temperature
We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using EDC carbodiimide crosslinker chemistry. Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22890), carboxylates react with amino (–NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry).
Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
Review of PEGylation
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins