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EZ-Link Amine-PEG-Biotin Reagents


Short, hydrophilic reagents for biotinylation of carboxyls via EDC.

EZ-Link Amine-PEG2-Biotin

Thermo Scientific EZ-Link Amine-PEG2-Biotin and Amine-PEG3-Biotin are water-soluble biotin compounds containing polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods.

Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the –NH2 group of the amino-biotin, forming an amide bond.

Highlights:

  • Biotinylation – label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins
  • Amine-activated – primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers
  • Pegylated – polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules
  • Medium length – spacer arms (total lengths added to target) are 20.4 and 22.9 angstroms, providing slightly different options for controlling steric hindrance and hydrophilicity

Product Details:

Chemical structure of Amine-PEG2-Biotin

Chemical structure of Amine-PEG3-Biotin

Chemical structures of Amine-PEG-Biotins. These biotinylation reagents are often conjugated to carboxyl groups using the crosslinker EDC. For more information, see our review of Carbodiimide Reaction Chemistry.

Properties of Amine-PEG-Biotin Compounds. Also see Amine-PEG11-Biotin.
  Amine-PEG2-Biotin Amine-PEG3-Biotin
Alternative names Biotin-PEO2-Amine
Biotin-dPEG2-Amine
Biotin-PEO3-Amine
Biotin-dPEG3-Amine
Chemical name (+)-biotinyl-3,6-dioxaoctanediamine (+)-biotinyl-3,6,9,-trioxaundecanediamine
Molecular formula C16H30N4O4S C18H34N4O5S
Molecular weight 374.50 418.55
Spacer arm length 20.4 angstroms 22.9 angstroms
Mass added to target 356.19 daltons 400.21 daltons
Form White to off-white solid White to off-white solid
Solubility At least 25mg/mL in water At least 25mg/mL in water
Storage conditions 4°C 4°C
SMILES structures NCCOCCOCCNC(CCCCC1C(C(CS1)N2)NC2=O)=O
O=C1NC2C(C(CCCCC(NCCOCCOCCOCCN)=O)SC2)N1

We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.

Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using EDC carbodiimide crosslinker chemistry. Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22890), carboxylates react with amino (–NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry).

References:

  1. Bronfman, F.C., et al. (2003) J. Neurosci.. 23, 3209-3220.
  2. Hara, T., et al. (2003) J. Biol. Chem. 278, 40408-40414.
  3. Pihlajamaa, T., et al. (2004) J. Biol. Chem.279, 24265-24273.

Related Resources:

Biotinylation Reagents Selection Guide
Review of Biotinylation Methods and Applications
Chemistry of Crosslinking (and Labeling Reagents)
Review of PEGylation

Related Products:

EDC Crosslinker
Sulfo-NHS
Amine-PEG11-Biotin
All Biotinylation Reagents
Avidin, Streptavidin, NeutrAvidin Affinity Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21346 EZ-Link Amine-PEG2-Biotin
Formulation: Diethyleneglycol with amine and biotin ends
Sufficient For: Approx. 25 uses to label a total of 45mg of IgG using typical conditions with EDC crosslinker
50mg Product Instructions for product #21346 EZ-Link Amine-PEG2-Biotin MSDS for product #21346 EZ-Link Amine-PEG2-Biotin Certificate of Analysis for product #21346 EZ-Link Amine-PEG2-Biotin $235.00

Add to cart
21346B EZ-Link Amine-PEG2-Biotin
Formulation: Diethyleneglycol with amine and biotin ends
Custom MSDS for product #21346B EZ-Link Amine-PEG2-Biotin Certificate of Analysis for product #21346B EZ-Link Amine-PEG2-Biotin
21347 EZ-Link Amine-PEG3-Biotin
Formulation: Triethyleneglycol with amine and biotin ends
Sufficient For: Approx. 25 uses to label a total of 40mg of IgG using typical conditions with EDC crosslinker
50mg Product Instructions for product #21347 EZ-Link Amine-PEG3-Biotin MSDS for product #21347 EZ-Link Amine-PEG3-Biotin Certificate of Analysis for product #21347 EZ-Link Amine-PEG3-Biotin $250.00

Add to cart
21347B EZ-Link Amine-PEG3-Biotin
Formulation: Triethyleneglycol with amine and biotin ends
Custom MSDS for product #21347B EZ-Link Amine-PEG3-Biotin Certificate of Analysis for product #21347B EZ-Link Amine-PEG3-Biotin

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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