Thermo Scientific Pierce Ethylenediamine Dihydrochloride is a simple compound that can be used with EDC (carbodiimide) crosslinker to polymerize or amine-modify carboxylate molecules for a variety of applications.
Ethylenediamine Dihydrochloride is composed of two amines separated by a short hydrocarbon spacer. This versitle reagent is used for a variety of applications, including covalent modification of carboxylic acids to block the carboxylates and form terminal amino groups. Such amino groups can then be used for a variety of conjugation strategies. Furthermore, oligonucleotides can be amine-modified for labeling with biotin or fluorophores or immobilized onto a solid support using amine conjugation chemistries (see Tech Tip # 30).
Structure and properties of ethylenediamine:
- Functional groups: primary amines (both ends)
- Chemical name: ethylenediamine dihydrochloride
- Synonyms: 1,2-ethanediamine; 1,2-diaminoethane
- Chemical formula: C2H8N2•2HCl
- Molecular weight: 133.02
- CAS number: 107-15-3
- Form: crystals, free of foreign matter
- Purity: greater than 98%
Overview of crosslinking and protein modification