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Equine TNF alpha ELISA Kits

Reagent kit for sandwich ELISA of horse TNF alpha (equine TNFa).

Thermo Scientific Equine TNF alpha ELISA Kits

Assay TNFα (equine tumor necrosis factor alpha, TNF alpha) levels in horse cell culture supernatant samples with our Thermo Scientific Equine TNF alpha ELISA Kits.

This equine TNF alpha ELISA reagent kit uses pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of horse tumor necrosis factor alpha (TNFα) in samples at 100μL per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The economically-priced reagent kit provides validated reagents only, requiring users to coat their own plates. All components undergo quality control testing to minimize lot-to-lot variability.


  • Target: natural and recombinant horse TNFα (equine tumor necrosis factor alpha)
  • Format: colorimetric ELISA (TMB substrate) for 96-well microplates
  • Measurement: absorbance at 450nm minus absorbance at 550nm
  • Assay Range: 16 to 1000pg/mL
  • Sensitivity: undetermined
  • Sample Types: cell culture supernatant
  • Total Assay Time: 3 hours
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with bovine, mouse, or rat TNFα. Partial cross-reaction occurs with human and porcine TNFα (< 10%).
Video of the Thermo Scientific TNF alpha ELISA Kit


The Reagent Kit (previously called a "Screening Set") contains matched antibodies, standard and detection reagents but no coated plates.


Horse TNF alpha ELISA standard curve
Equine TNF alpha ELISA standard curve. An example standard curve for the Thermo Scientific Equine TNF alpha ELISA Reagent Kit (Part No. ESS0017), with error bars based on six replicates for each tumor necrosis factor alpha standard concentration.


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This product is for In Vitro experimental use only. Not for resale without express authorization.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
ESS0017 Equine TNF alpha ELISA Reagent Kit
Formulation: Antibodies and complete reagent set for ELISA
Sufficient For: 5 x 96 microplate wells

Kit Contents:
TNF alpha Coating Antibody, 0.625mL
TNF alpha Detection Antibody, 0.625mL
Equine TNF alpha Standard, 5 vials
Streptavidin-HRP Conjugate, 0.25mL
TMB Substrate Solution, 55mL
Stop Solution, 55mL
5-plate kit Product Instructions for product #ESS0017 Equine TNF alpha ELISA Reagent Kit MSDS for product #ESS0017 Equine TNF alpha ELISA Reagent Kit $425.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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