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An amine-to-sulfhydryl crosslinker with a medium-length spacer arm (9.4 angstroms).

Pierce EMCS

Thermo Scientific Pierce EMCS is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).


  • Reactive groups: NHS ester and maleimide
  • Reactive towards: amino and sulfhydryl groups
  • C6-homolog of aliphatic spacer series which includes AMAS, BMPS, GMBS and EMCS
  • NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds
  • Maleimides react with -SH groups at pH 6.5-7.5, forming stable thioether linkages
  • Non-cleavable
  • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EMCS
  • Increased sphere of coupling vs. GMBS
  • Short aliphatic spacer has low potential for eliciting an immune response

Product Details:

N-epsilon-Malemidocaproyl-oxysuccinimide ester; EMCS
Chemical structure of EMCS crosslinking reagent.


  1. Fujiwara, K., et al. (1988). J. Immunol. Methods 112, 77-83.
  2. Peeters, J.M., et al. (1989). J. Immunol. Methods 120, 133-143.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22308 EMCS
Formulation: N-epsilon-Malemidocaproyl-oxysuccinimide ester; powder solid
Sufficient For: 162mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22308 EMCS MSDS for product #22308 EMCS Certificate of Analysis for product #22308 EMCS $213.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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