Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.
- Reactive groups: NHS ester (both ends)
- Reactive towards: amino groups (primary amines)
- Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
- Membrane-permeable, allowing for intracellular crosslinking
- Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
- Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS
|Chemical structure of EGS crosslinking reagent.
- Millar, J.B. and Rozengur, E. (1990). Chronic desensitization to bombesin by progressive down-regulation of bombesin receptors in Swiss 3T3 cells. J. Biol. Chem. 265, 12052-12058.
- Browning, J. and Ribolini, A. (1989). Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines. J. Immunol. 143, 1859-1867.
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