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EGS (Ethylene glycol bis[succinimidylsuccinate])


A long-arm crosslinker that is cleavable with hydroxylamine.

Pierce EGS

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Highlights:

  • Reactive groups: NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
  • Membrane-permeable, allowing for intracellular crosslinking
  • Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
  • Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product Details:

Ethylene glycol bis(succinimidylsuccinate); EGS
 
Chemical structure of EGS crosslinking reagent.

References:

  1. Millar, J.B. and Rozengur, E. (1990). Chronic desensitization to bombesin by progressive down-regulation of bombesin receptors in Swiss 3T3 cells. J. Biol. Chem. 265, 12052-12058.
  2. Browning, J. and Ribolini, A. (1989). Studies on the differing effects of tumor necrosis factor and lymphotoxin on the growth of several human tumor lines. J. Immunol. 143, 1859-1867.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
Sulfo-EGS (water-soluble)
Hydroxylamine-HCl


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21565 EGS
Formulation: Ethylene glycol bis(succinimidylsuccinate); powder solid
Sufficient For: 2191mL of typical 1X (1mM) reaction solution, or approx. 500 uses at 2mg reagent per use
1g Product Instructions for product #21565 EGS MSDS for product #21565 EGS Certificate of Analysis for product #21565 EGS $159.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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