Thermo Scientific Pierce Premium Grade EDC is our highest quality formulation of this popular carbodiimide crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.
Compared to the standard grade product, Premium Grade EDC provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. EDC is 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride, a water-soluble carbodiimide crosslinker that activates carboxyl groups for spontaneous reaction with primary amines, enabling peptide immobilization and hapten-carrier protein conjugation. In the presence of N-hydroxysulfosuccinimide (sulfo-NHS), EDC can be used to convert carboxyl groups to amine-reactive sulfo-NHS esters. This is accomplished by mixing the EDC with a carboxyl containing molecule and adding sulfo-NHS.
- EDC – carbodiimide crosslinker for conjugation between carboxylates and amines
- High quality – identity and purity confirmed by several tests
- Product integrity – enhanced level of testing and characterization compared to standard grade
- Lot retention – ample supply of past lots retained to ensure future process testing
- Change management – Change Control Notification (CCN) service
- Consistent manufacture – batch-specific manufacturing documentation review
|Chemical structure of EDC crosslinking reagent. For more complete information about the chemical properties and uses of this reagent, see the product page for standard grade EDC.
Specifications of Premium Grade EDC. We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
||Premium Grade Specification
||White crystalline powder, free of foreign matter
||IR scan shows only peaks characteristic of EDC
||Karl Fischer titration, ≤ 2%
||All metals, including lead, ≤ 50 ppm
||Reported values for C, H, and N
Crosslinker Application Guide -- search for recent literature references for this product
Overview of crosslinking
Chemistry of crosslinking
Premium Grade Reagents – complete menu
EDC (standard grade)
Sulfo-NHS (Premium Grade)
Sulfo-NHS (standard grade)
Crosslinkers at a glance (selection guide)