Update profile
    Change password
    Sign out

Easy-Titer Antibody Assay Kits


Measure antibody concentration in serum and culture supernatant samples.

Easy-Titer Antibody Assay Kits

Thermo Scientific Easy-Titer Antibody Assay Kits enable accurate determination of antibody concentration in serum and culture supernatant, and include kits specific for mouse IgG, rabbit IgG, human IgG (H+L), human IgG (Fc), or human IgM.

Easy-Titer Kits include antibody-sensitized microspheres to measure the specific concentration of antibodies by an easy and rapid microagglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). Each kit is specific for a particular species and class of immunoglobulin and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. The kits are extremely sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

Highlights:

  • Kits for five popular targets – choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody
  • Antibody-based specificity – measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
  • Faster and easier than ELISA – three-component, homogenous assay; 10 minutes total incubation time
  • No special equipment needed – uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
  • Sensitive – assay range (standard curve) 8 to 500ng/mL; requires starting sample of at least 300ng/mL
  • Reproducible – coefficient of variation < 5%; error depends on dilution and pipetting technique
  • Antibody standards sold separately – see product list for suggested products; use any antibody standard with proper target identity and known concentration (greater than 10µg/mL)

Product Details:

Easy-Titer Assay Kits detect and measure specific target antibodies using agglutination of microspheres that are coated ("sensitized") with the specific anti-IgG or IgM polyclonal antibodies. In the appropriate aqueous buffer (supplied in kit), the monodispersed antibody-coated microspheres (> 1µm diameter) have highest absorptivity (λ-max) to incident light having a wavelength (340nm) that is equal to approximately half their diameter. When sample is added, two or more microspheres bind to each antibody target via their coated specific polyclonal antibodies, and this agglutination into effectively larger apparent spheres results in proportional decrease in absorptivity (lower absorbance).

Easy-Titer Assay Kits use microagglutination to specifically detect target antibodies.
Microsphere agglutination assay. Thermo Scientific Easy-Titer Assay Kits use antibody-sensitized microspheres to specifically assay target antibody species based on microagglutination.

 

Easy-Titer Assay Kit results plotted from 340nm or 405nm absorbance measurements. Antibody quantitation by microagglutination assay. Easy-Titer Assay Kit results, plotted from 340nm or 405nm absorbance measurements, yield negative-slope standard curves for assessment of diluted samples in the range 8 to 500ng/mL.

Typical microagglutination assays depend on a change in light-scattering and corresponding change in transmittance, to which absorbance is inversely related. Easy-Titer Assay Kits use a special dilution buffer whose refractive index eliminates the effect of light-scattering on the monodispersed microspheres for the measurement wavelength used. Because of this, the final 10- to 20-fold dilution of the sample for use in the assay must be done using the Dilution Buffer supplied in the kit.

Easy-Titer Antibody Assays are faster and easier than ELISA:

  1. Prepare standards (5 to 500ng/mL) by diluting purified antibody in Kit Dilution Buffer.
  2. Prepare samples by diluting in Dilution Buffer to within assay range (8 to 500ng/mL).
  3. Vortex vial of microsphere beads to create homogeneous suspension.
  4. Pipette 20µL of bead suspension and 20µL of each sample and standard into 96-well microplate wells.
  5. Incubate microplate for 5 minutes with vigorous mixing.
  6. Add 100µL of Kit Blocking Reagent.
  7. Incubate microplate for 5 minutes with vigorous mixing.
  8. Measure absorbance on standard plate reader (340nm or 405nm).
  9. Plot standard curve and interpolate samples to determine concentration.

Related Resources:

Review of antibody screening, isotyping and quantitation methods

Related Products:

Mouse IgG (Part No. 31903) – use as standard for Part No. 23300
Rabbit IgG (Part No. 31235) – use as standard for Part No. 23305
Human IgG (Part No. 31154) – use as standard for Part No. 23310, 23325
Human IgM (Part No. 31146) – use as standard for Part No. 23315
Other antibody isotype controls
96-Well Plates


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
23300 Easy-Titer Mouse IgG Assay Kit
Formulation: Specific for all subclasses of mouse IgG
Sufficient For: 96 total tests (including 9 standards per use)

Kit Contents:
Goat Anti-Mouse IgG Sensitized Beads, 2mL
Easy-Titer Dilution Buffer, 30mL
Easy-Titer Blocking Buffer, 15mL
96-test kit Product Instructions for product #23300 Easy-Titer Mouse IgG Assay Kit MSDS for product #23300 Easy-Titer Mouse IgG Assay Kit Certificate of Analysis for product #23300 Easy-Titer Mouse IgG Assay Kit $314.00

Add to cart
23305 Easy-Titer Rabbit IgG Assay Kit
Formulation: Specific for all subclasses of rabbit IgG
Sufficient For: 96 total tests (including 9 standards per use)

Kit Contents:
Goat Anti-Rabbit IgG Sensitized Beads, 2mL
Easy-Titer Dilution Buffer, 30mL
Easy-Titer Blocking Buffer, 15mL
96-test kit Product Instructions for product #23305 Easy-Titer Rabbit IgG Assay Kit MSDS for product #23305 Easy-Titer Rabbit IgG Assay Kit Certificate of Analysis for product #23305 Easy-Titer Rabbit IgG Assay Kit $309.00

Add to cart
23310 Easy-Titer Human IgG (H+L) Assay Kit
Formulation: Specific for all subclasses, fragments and chains of human IgG
Sufficient For: 96 total tests (including 9 standards per use)

Kit Contents:
Goat Anti-Human IgG Sensitized Polystyrene Beads, 2mL
Easy-Titer Dilution Buffer, 30mL
Easy-Titer Blocking Buffer, 15mL
96-test kit Product Instructions for product #23310 Easy-Titer Human IgG (H+L) Assay Kit MSDS for product #23310 Easy-Titer Human IgG (H+L) Assay Kit Certificate of Analysis for product #23310 Easy-Titer Human IgG (H+L) Assay Kit $339.00

Add to cart
23325 Easy-Titer Human IgG (gamma chain) Assay Kit
Formulation: Specific for the Fc fragment of all subclasses of human IgG
Sufficient For: 96 total tests (including 9 standards per use)

Kit Contents:
Goat Anti-Human IgG (gamma chain) Sensitized Polystyrene Beads, 2mL
Easy-Titer Dilution Buffer, 30mL
Easy-Titer Blocking Buffer, 15mL
96-test kit Product Instructions for product #23325 Easy-Titer Human IgG (gamma chain) Assay Kit MSDS for product #23325 Easy-Titer Human IgG (gamma chain) Assay Kit Certificate of Analysis for product #23325 Easy-Titer Human IgG (gamma chain) Assay Kit $351.00

Add to cart
23315 Easy-Titer Human IgM Assay Kit
Formulation: Specific for all human IgM
Sufficient For: 96 total tests (including 9 standards per use)

Kit Contents:
Goat Anti-Human IgM Sensitized Polystyrene Beads, 2mL
Easy-Titer Dilution Buffer, 30mL
Easy-Titer Blocking Buffer, 15mL
96-test kit Product Instructions for product #23315 Easy-Titer Human IgM Assay Kit MSDS for product #23315 Easy-Titer Human IgM Assay Kit Certificate of Analysis for product #23315 Easy-Titer Human IgM Assay Kit $349.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
23209 - Bovine Serum Albumin Standard Ampules, 2mg/mL

66380 - Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3mL

23227 - BCA Protein Assay Kit

23225 - BCA Protein Assay Kit

66810 - Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12mL

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747