Label peptides, antibodies and other proteins at primary amines with rhodamine-based Thermo Scientific DyLight Green-emitting Specialty Dye, which is comparable to Alexa Fluor 546, Alexa Fluor 555 and Cy3.
DyLight Yellow-emitting Dyes are a family of labeling agents that provide bright fluorescent detection for imaging. Dyes can be selected based upon their characteristic excitation and emission properties or relative hydrophilicity and hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.
- NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
- Multiple solubility options – choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application
Criteria to consider when choosing a DyLight Yellow-emitting Specialty Dye:
- Excitation and emission wavelengths – choose the best dye to match the excitation and emission capabilities of your instrument
- Water solubility – choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
- Fluorescence imaging
- Fluorescence correlation spectroscopy
- Flow cytometry
- Antibody labeling
- Direct immunofluorescence staining
- Western blotting
- Protein microarrays
- Polymer labeling
- Peptide labeling
- Phalloidin labeling for actin staining
Thermo Scientific DyLight Yellow-Emitting Specialty Dye Characteristics.
||Laser and filter sets
||Rhodamine, 0 sulfonates
||546 laser source
532 flow cytometry laser line
Cy3 filter set
546 mercury arc emission
||Rhodamine, 1 sulfonate
|† Fluorescent dye name appended with abbreviated core structure (e.g., R1: Rhodamine with 1 sulfonate)
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum
Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide
DyLight 622Q-NHS Ester (complementary fluorescence quencher)
DyLight 550 Amine-Reactive Dye and Kits
TRITC and Rhodamine Dyes and Labeling Kits