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DyLight In Vivo Apoptosis Detection Kits


Nontoxic, cell-permeable probes for the selective detection of active caspases.

DyLight In Vivo Apoptosis Detection Kits

Thermo Scientific DyLight 690 and DyLight 747 In Vivo Apoptosis Detection Kits offer an accurate method to detect apoptosis in small-animal imaging.

These kits contain a DyLight 690- or DyLight 747-conjugated in vivo polycaspase probe, which is a cell-permeable fluorescent detector of active caspases indicative of apoptosis. The probes are comprised of a polycaspase peptide sequence (VAD) and a fluoromethyl ketone (FMK) moiety, which facilitate irreversible binding of the probe to active caspases, and either DyLight 690 or DyLight 747 Dye for near-infrared detection with a range of in vivo instrumentation. These probes are valuable for in vivo drug determination studies, because changes in apoptosis from drug reactivity can be measured by fluorescence imaging in live animals. An increase in apoptosis produces a greater attachment of probes and, subsequently, an increase in fluorescence output. The in vivo technique is nondestructive, thus eliminating the need to remove cells and tissues from sacrificed animals prior to analysis (which induces further apoptosis). Additionally, apoptosis can be monitored in a single animal at multiple time points, reducing the number of animals required for a given study.

Highlights:

  • Nontoxic – not harmful to valuable research animals
  • Cell-permeable – readily diffuses through all cells as it circulates throughout the body
  • Selective for active caspases – eliminates high background and false-positive results common with other caspase detection methods

Applications:

  • Small-animal in vivo apoptosis imaging

Product Details:

For optimal results, use DyLight 690 In Vivo Control Reagent (Product No. 84889) or DyLight 747 In Vivo Control Reagent (Product No. 84896) to assess the binding specificity of the polycaspase probe for the apoptotic cells or tissue within the animal. Two control groups are recommended: one group of untreated animals that are injected with the polycaspase probe; and one group of treated animals that are injected with the representative DyLight In Vivo Control Reagent.

Properties of the Thermo Scientific DyLight Polycaspase Probes

Fluorescent Dye

Ex/Em

円

MW†††

DyLight 690

690/709

141K

950

DyLight 747

747/776

223K

1028

†     Excitation and emission maxima in nanometers (±4nm)
††   Molar extinction coefficient (M-1 cm-1) at the absorption maximum
††† Molecular weight (g/mol) of the probe


DyLight 680 and 747 In Vivo Polycaspase Probe Data

Thermo Scientific DyLight 690 and 747 Apoptosis Detection Kits offer non-invasive active caspase detection. Adult wild-type Balb/c mice were mock-infected or inoculated intranasally with Herpes simplex virus-1 (HSV1), which is known to induce apoptosis in the brain. Mice were then injected intravenously with DyLight 690 (top panel) or 747 (bottom panel) In Vivo Polycaspase Probe 7 days post-infection. In the second experiment, DyLight 747 In Vivo Control Reagent was injected into an HSV1-infected mouse as a reagent control. After 4 or 7 hours (top and bottom panels, respectively), all animals were imaged using a Carestream* In Vivo FX PRO* imager. Strong caspase activity was detected in the brains of animals treated with HSV-1 and injected with either DyLight 690 or 747 caspase probe but not in mock-infected or reagent control animals. Minimal signal was detected in the liver region of the HSV-treated animal injected with either caspase probe, indicating minimal clearance of the probe. Strong signal intensity in the tail indicates where reagent is likely to pool after IV injection.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
84888 DyLight 690 In Vivo Apoptosis Detection Kit
Formulation: Multi-component kit with DyLight 690-conjugated caspase inhibitor modified with a fluoromethyl ketone (FMK)
Sufficient For: Injecting approx. 10 mice

Kit Contents:
DyLight 690 In Vivo Polycaspase Probe, 1 vial
10X Injection Buffer, 5mL
10-test kit Product Instructions for product #84888 DyLight 690 In Vivo Apoptosis Detection Kit MSDS for product #84888 DyLight 690 In Vivo Apoptosis Detection Kit Certificate of Analysis for product #84888 DyLight 690 In Vivo Apoptosis Detection Kit $416.00

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84889 DyLight 690 In Vivo Control Reagent
Formulation: Proprietary dark green lyophilized powder
Sufficient For: 10 tests per vial
1 vial MSDS for product #84889 DyLight 690 In Vivo Control Reagent Certificate of Analysis for product #84889 DyLight 690 In Vivo Control Reagent $259.00

Add to cart
84895 DyLight 747 In Vivo Apoptosis Detection Kit
Formulation: Multi-component kit with DyLight 747-conjugated caspase inhibitor modified with a fluoromethyl ketone (FMK)
Sufficient For: Injecting approx. 10 mice

Kit Contents:
DyLight 747 In Vivo Polycaspase Probe, 1 vial
10X Injection Buffer, 5mL
10-test kit Product Instructions for product #84895 DyLight 747 In Vivo Apoptosis Detection Kit MSDS for product #84895 DyLight 747 In Vivo Apoptosis Detection Kit Certificate of Analysis for product #84895 DyLight 747 In Vivo Apoptosis Detection Kit $416.00

Add to cart
84896 DyLight 747 In Vivo Control Reagent
Formulation: Proprietary dark green lyophilized powder
Sufficient For: 10 tests per vial
1 vial MSDS for product #84896 DyLight 747 In Vivo Control Reagent Certificate of Analysis for product #84896 DyLight 747 In Vivo Control Reagent $259.00

Add to cart
84897 10X Injection Buffer
Formulation: Proprietary in vivo caspase probe injection buffer
Sufficient For: Preparing 50mL of 1X Injection Buffer
5mL MSDS for product #84897 10X Injection Buffer Certificate of Analysis for product #84897 10X Injection Buffer $51.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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