Perform FRET-based assays with benzopyrillium-, azo- and benzocyanine-based Thermo Scientific DyLight Quencher Specialty Dyes, which effectively quench matching DyLight and Alexa Fluor dyes.
DyLight Quencher Specialty Dyes are a family of non-fluorescent dyes designed for fluorescence quenching in FRET applications. The optimal quencher dye can be selected based upon its characteristic absorption properties that match the emission of a corresponding fluor. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.
- Large selection – the largest family of dyes available for far red-emitting fluorescence applications
- NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
Criteria to consider when choosing a DyLight Quencher Specialty Dye:
- Absorption wavelength – choose the quencher dye with the absorbance wavelength that matches the emission of your fluorophore and the instrument.
- Water solubility – choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
- DyLight dye selection – the broad selection of red-emitting dyes allows a number of candidate dyes to be tested in an application for optimal performance.
- Other quenching applications
Thermo Scientific DyLight Quencher Specialty Dye Characteristics.
||Laser and filter sets
||DyLight 350, DyLight 405
||DY-415-Co1, DyLight 488, DEAC
||DyLight 488, DyLight 556-R1,
||DyLight 556-R1, DyLight 590-R2,
DyLight 633-B3, DyLight 651-B1
||DyLight 633-B2, DyLight 633, DyLight 655-B3,
||DyLight 680, DyLight 750
|† Fluorescent dye name appended with Q to indicate that it is a quencher dye
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum
- Tan C. et al. (2010) Direct detection of Delta9-tetrahydrocannabinol in saliva using a novel homogeneous competitive immunoassay with fluorescence quenching. Anal Chim Acta. 658, 187-92.
Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide
All DyLight Fluor Labeling Reagents and Kits