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DyLight Quencher Specialty Dyes


Quenching labels to pair with DyLight and Alexa Fluor dyes for FRET applications.

DyLight Quencher Specialty Dyes

Perform FRET-based assays with benzopyrillium-, azo- and benzocyanine-based Thermo Scientific DyLight Quencher Specialty Dyes, which effectively quench matching DyLight and Alexa Fluor dyes.

DyLight Quencher Specialty Dyes are a family of non-fluorescent dyes designed for fluorescence quenching in FRET applications. The optimal quencher dye can be selected based upon its characteristic absorption properties that match the emission of a corresponding fluor. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.

Highlights:

  • Large selection – the largest family of dyes available for far red-emitting fluorescence applications
  • NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation

Criteria to consider when choosing a DyLight Quencher Specialty Dye:

  • Absorption wavelength – choose the quencher dye with the absorbance wavelength that matches the emission of your fluorophore and the instrument.
  • Water solubility – choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
  • DyLight dye selection – the broad selection of red-emitting dyes allows a number of candidate dyes to be tested in an application for optimal performance.

Applications:

  • FRET
  • Other quenching applications

Product Details:

DyLight Quencher Specialty Dye benzopyrillium core structure

Thermo Scientific DyLight Quencher Specialty Dye Characteristics.
Fluorescent Dye λmax
(nm)
Solvent 円 Core structure Laser and filter sets
DyLight 425Q
425 PBS 25K Azo DyLight 350, DyLight 405
DyLight 504Q
504 PBS 39K Azo DY-415-Co1, DyLight 488, DEAC
DyLight 543Q
544 PBS 44K Azo DyLight 488, DyLight 556-R1,
DyLight 590-R2, DyLight 633-B3,
DyLight 651-B1
DyLight 641Q
641 Ethanol 105K Benzopyrillium DyLight 556-R1, DyLight 590-R2,
DyLight 633-B3, DyLight 651-B1
DyLight 662Q
661 Ethanol 140K Benzopyrillium DyLight 515-LS
DyLight 683Q
680 PBS 80K Benzocyanine DyLight 633-B2, DyLight 633, DyLight 655-B3, DyLight 655-B4,
DyLight 680
DyLight 696Q
696 PBS 49K Benzocyanine DyLight 680B
DyLight 766Q
766 Ethanol 180K Benzopyrillium DyLight 680, DyLight 750
†   Fluorescent dye name appended with Q to indicate that it is a quencher dye
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum

References:

  • Tan C. et al. (2010) Direct detection of Delta9-tetrahydrocannabinol in saliva using a novel homogeneous competitive immunoassay with fluorescence quenching. Anal Chim Acta. 658, 187-92.

Related Resources:

Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide

Related Products:

All DyLight Fluor Labeling Reagents and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
84508 DyLight 543Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Azo quencher
1mg Product Instructions for product #84508 DyLight 543Q NHS Ester MSDS for product #84508 DyLight 543Q NHS Ester Certificate of Analysis for product #84508 DyLight 543Q NHS Ester $213.00

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84509 DyLight 641Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Benzopyrillium quencher
1mg Product Instructions for product #84509 DyLight 641Q NHS Ester MSDS for product #84509 DyLight 641Q NHS Ester Certificate of Analysis for product #84509 DyLight 641Q NHS Ester $213.00

Add to cart
84510 DyLight 683Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Acridinium quencher
1mg Product Instructions for product #84510 DyLight 683Q NHS Ester MSDS for product #84510 DyLight 683Q NHS Ester Certificate of Analysis for product #84510 DyLight 683Q NHS Ester $213.00

Add to cart
84511 DyLight 766Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Benzopyrillium quencher
1mg Product Instructions for product #84511 DyLight 766Q NHS Ester MSDS for product #84511 DyLight 766Q NHS Ester Certificate of Analysis for product #84511 DyLight 766Q NHS Ester $213.00

Add to cart
84512 DyLight 425Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Azo quencher
1mg Product Instructions for product #84512 DyLight 425Q NHS Ester MSDS for product #84512 DyLight 425Q NHS Ester Certificate of Analysis for product #84512 DyLight 425Q NHS Ester $213.00

Add to cart
84513 DyLight 504Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Azo quencher
1mg Product Instructions for product #84513 DyLight 504Q NHS Ester MSDS for product #84513 DyLight 504Q NHS Ester Certificate of Analysis for product #84513 DyLight 504Q NHS Ester $213.00

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84514 DyLight 662Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Benzopyrillium quencher
1mg Product Instructions for product #84514 DyLight 662Q NHS Ester MSDS for product #84514 DyLight 662Q NHS Ester Certificate of Analysis for product #84514 DyLight 662Q NHS Ester $213.00

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84515 DyLight 696Q NHS Ester
Formulation: Non-fluorescent NHS ester activated Azo quencher
1mg Product Instructions for product #84515 DyLight 696Q NHS Ester MSDS for product #84515 DyLight 696Q NHS Ester Certificate of Analysis for product #84515 DyLight 696Q NHS Ester $213.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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