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DyLight Near Infrared-emitting Specialty Dyes


Label molecules with intensely fluorescent near infrared dyes for imaging.

DyLight Near Infrared Specialty Dyes

Label peptides, antibodies and other proteins at primary amines with benzopyrillium- and cyanine-based Thermo Scientific DyLight Near Infrared Specialty Dyes, which are comparable to Alexa Fluor and IRDye NIR dyes.

DyLight NIR Dyes are a family of labeling agents that can be used for bright fluorescence detection in cell-based imaging or in vivo imaging applications. NIR dyes can be selected based upon their characteristic excitation and emission properties or relative hydrophilicity and hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation. NIR dyes are best for imaging through tissues and away from indigenous fluorescent biomolecule interference or quenching. DyLight Near Infrared Dyes represent the largest selection of fluorescent labels that are commercially available.

Highlights:

  • Large selection – the largest family of dyes available for NIR fluorescence applications
  • NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
  • Broad spectrum of water solubilities – choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application
  • NIR dyes avoid background interference – DyLight NIR Dyes avoid fluorescence interference or quenching effects from biomolecules present in samples
  • Excellent signal penetration through cells and tissues – DyLight NIR Dyes provide the optimal window for excitation and emission for in vivo imaging applications

Criteria to consider when choosing a DyLight NIR Specialty Dye:

  • Excitation and emission wavelengths – choose the best dye to match the excitation and emission capabilities of your instrument
  • Water solubility – choose a DyLight NIR Dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
  • DyLight Dye selection – the broad selection of NIR dyes allows a number of candidate dyes to be tested in a given application for optimal performance.

Applications:

  • In vivo or ex vivo imaging
  • Tumor imaging with labeled peptides
  • NIR fluorescence (NIRF) imaging of labeled silica nanoparticles
  • NIR in vitro imaging and characterization
  • Determination of thermal stability
  • Cytotoxicity assays
  • Molecular imaging
  • UV-VIS-NIR spectroscopy
  • Fluorescence correlation spectroscopy
  • MRI applications
  • DNA sequencing
  • Primer labeling for PCR
  • 2-D gel electrophoresis
  • Flow cytometry/fluorescence-activated cell sorting (FACS)
  • Laser scanning confocal microscopy

Product Details:

DyLight Near Infrared Specialty Dye benzopyrillium core structure DyLight Near Infrared Specialty Dye cyanine core structure

 

Thermo Scientific DyLight Near Infrared Specialty Dye Characteristics.
Fluorescent Dye Ex/Em (nm) Solvent 円 Core structure Laser and filter sets
DyLight 675-B1
675/699 Ethanol 180K Benzopyrillium, 1 sulfonate 633 laser source
Far red diode laser
Cy 5.5 filter set
Licor Odyssey and Aerius (700 channel)
Other near IR Imaging systems
DyLight 675-B2
675/699 Ethanol 180K  Benzopyrillium, 2 sulfonates
DyLight 675-B3
674/698 Ethanol 180K  Benzopyrillium, 3 sulfonates
DyLight 675-B4
673/694 Ethanol 180K  Benzopyrillium, 4 sulfonates
DyLight 679-C5
679/698 PBS 200K Cyanine, 5 sulfonates
DyLight 690-B1
691/709 Ethanol 140K  Benzopyrillium, 1 sulfonate
DyLight 690-B2
692/709 Ethanol 140K  Benzopyrillium, 2 sulfonates
DyLight 700-B1
707/728 Ethanol 140K  Benzopyrillium, 1 sulfonate 633 laser source
Licor Odyssey and Aerius (700 channel)
Other near IR Imaging systems
DyLight 700-B2
709/730 Ethanol 140K  Benzopyrillium, 2 sulfonates
DyLight 730-B1
734/755 Ethanol 240K Benzopyrillium, 1 sulfonate
DyLight 730-B2
736/755 Ethanol 240K  Benzopyrillium, 2 sulfonates
DyLight 730-B3
736/755 Ethanol 205K Benzopyrillium, 3 sulfonates
DyLight 730-B4
733/755 Ethanol 240K  Benzopyrillium, 4 sulfonates
DyLight 747-B1
751/774 Ethanol 270K  Benzopyrillium, 1 sulfonate 633 laser source
Xenon arc excitation
Licor Odyssey and Aerius (700 channel)
Other near IR Imaging systems
DyLight 747-B2
751/774 Ethanol 270K  Benzopyrillium, 2 sulfonates
DyLight 747-B3
750/771 Ethanol 270K  Benzopyrillium, 3 sulfonates
DyLight 747-B4
748/775 Ethanol 270K  Benzopyrillium, 4 sulfonate
DyLight 775-B2
772/787 Ethanol 240K   Benzopyrillium, 2 sulfonates
DyLight 775-B3
770/788 Ethanol 240K   Benzopyrillium, 3 sulfonates
DyLight 775-B4
767/787 Ethanol 240K   Benzopyrillium, 4 sulfonates
DyLight 780-B1
783/799 Ethanol 170K Benzopyrillium, 1 sulfonate
DyLight 780-B2
784/796 Ethanol 170K  Benzopyrillium, 2 sulfonates
DyLight 780-B3
785/794 Ethanol 170K  Benzopyrillium, 3 sulfonates
DyLight 830-B2
844/875 Ethanol 220K Benzopyrillium, 2 sulfonates Suitable IR Imaging Systems
†   Fluorescent dye name appended with abbreviated core structure (e.g., B2: Benzopyrillium with 2 sulfonates)
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum

References:

  • Napp J. et al. (2011) Optical imaging in vivo with a focus on paediatric disease: Technical progress, current preclinical and clinical applications and future perspectives. Pediatr Radiol. 41, 161-75.
  • Gong H. et al. (2010) In vivo imaging of xenograft tumors using an epidermal growth factor receptor-specific affibody molecule labeled with a near-infrared fluorophore. Neoplasia. 12, 139-49.
  • Pauli J. et al. (2009) An in vitro characterization study of new near infrared dyes for molecular imaging. Eur J Med Chem. 44, 3496-503.
  • Tanisaka H. et al. (2008) Near-infrared fluorescent labeled peptosome for application to cancer imaging. Bioconjug Chem. 19, 109-17.
  • Riederer I. M. et al. (2008) Serial protein labeling with infrared maleimide dyes to identify cysteine modifications. J Proteomics. 71, 222-30.
  • Lisy M. R. et al. (2007) Fluorescent bacterial magnetic nanoparticles as bimodal contrast agents. Invest Radiol. 42, 235-41.

Related Resources:

Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide

Related Products:

DyLight 680 Amine-Reactive Dye and Kits
DyLight 755 Amine-Reactive Dye and Kits
DyLight 800 Amine-Reactive Dye and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46646 DyLight 675-B1 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #46646 DyLight 675-B1 NHS Ester MSDS for product #46646 DyLight 675-B1 NHS Ester Certificate of Analysis for product #46646 DyLight 675-B1 NHS Ester $213.00

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46647 DyLight 675-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #46647 DyLight 675-B2 NHS Ester MSDS for product #46647 DyLight 675-B2 NHS Ester Certificate of Analysis for product #46647 DyLight 675-B2 NHS Ester $213.00

Add to cart
46648 DyLight 675-B3 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 3 sulfonate groups
1mg Product Instructions for product #46648 DyLight 675-B3 NHS Ester MSDS for product #46648 DyLight 675-B3 NHS Ester Certificate of Analysis for product #46648 DyLight 675-B3 NHS Ester $213.00

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46649 DyLight 675-B4 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 4 sulfonate groups
1mg Product Instructions for product #46649 DyLight 675-B4 NHS Ester MSDS for product #46649 DyLight 675-B4 NHS Ester Certificate of Analysis for product #46649 DyLight 675-B4 NHS Ester $213.00

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53003 DyLight 679-C5 NHS Ester
Formulation: NHS ester activated Cyanine with 5 sulfonate groups
1mg Product Instructions for product #53003 DyLight 679-C5 NHS Ester MSDS for product #53003 DyLight 679-C5 NHS Ester Certificate of Analysis for product #53003 DyLight 679-C5 NHS Ester $213.00

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53022 DyLight 690-B1 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #53022 DyLight 690-B1 NHS Ester MSDS for product #53022 DyLight 690-B1 NHS Ester Certificate of Analysis for product #53022 DyLight 690-B1 NHS Ester $213.00

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53023 DyLight 690- B1 Free Acid
Formulation: Free acid Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #53023 DyLight 690- B1 Free Acid MSDS for product #53023 DyLight 690- B1 Free Acid Certificate of Analysis for product #53023 DyLight 690- B1 Free Acid $213.00

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53026 DyLight 690-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53026 DyLight 690-B2 NHS Ester MSDS for product #53026 DyLight 690-B2 NHS Ester Certificate of Analysis for product #53026 DyLight 690-B2 NHS Ester $225.00

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53028 DyLight 700-B1 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #53028 DyLight 700-B1 NHS Ester MSDS for product #53028 DyLight 700-B1 NHS Ester Certificate of Analysis for product #53028 DyLight 700-B1 NHS Ester $213.00

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53030 DyLight 700-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53030 DyLight 700-B2 NHS Ester MSDS for product #53030 DyLight 700-B2 NHS Ester Certificate of Analysis for product #53030 DyLight 700-B2 NHS Ester $213.00

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53032 DyLight 730-B1 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #53032 DyLight 730-B1 NHS Ester MSDS for product #53032 DyLight 730-B1 NHS Ester Certificate of Analysis for product #53032 DyLight 730-B1 NHS Ester $213.00

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53033 DyLight 730-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53033 DyLight 730-B2 NHS Ester MSDS for product #53033 DyLight 730-B2 NHS Ester Certificate of Analysis for product #53033 DyLight 730-B2 NHS Ester $213.00

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53036 DyLight 730-B3 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 4 sulfonate groups
1mg Product Instructions for product #53036 DyLight 730-B3 NHS Ester MSDS for product #53036 DyLight 730-B3 NHS Ester Certificate of Analysis for product #53036 DyLight 730-B3 NHS Ester $213.00

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53037 DyLight 730-B4 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 4 sulfonate groups
1mg Product Instructions for product #53037 DyLight 730-B4 NHS Ester MSDS for product #53037 DyLight 730-B4 NHS Ester Certificate of Analysis for product #53037 DyLight 730-B4 NHS Ester $213.00

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46623 DyLight 747-B1 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #46623 DyLight 747-B1 NHS Ester MSDS for product #46623 DyLight 747-B1 NHS Ester Certificate of Analysis for product #46623 DyLight 747-B1 NHS Ester $236.00

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53040 DyLight 747-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53040 DyLight 747-B2 NHS Ester MSDS for product #53040 DyLight 747-B2 NHS Ester Certificate of Analysis for product #53040 DyLight 747-B2 NHS Ester $213.00

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53041 DyLight 747-B3 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 3 sulfonate groups
1mg Product Instructions for product #53041 DyLight 747-B3 NHS Ester MSDS for product #53041 DyLight 747-B3 NHS Ester Certificate of Analysis for product #53041 DyLight 747-B3 NHS Ester $213.00

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53042 DyLight 747-B4 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 4 sulfonate groups
1mg Product Instructions for product #53042 DyLight 747-B4 NHS Ester MSDS for product #53042 DyLight 747-B4 NHS Ester Certificate of Analysis for product #53042 DyLight 747-B4 NHS Ester $213.00

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53054 DyLight 775-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53054 DyLight 775-B2 NHS Ester MSDS for product #53054 DyLight 775-B2 NHS Ester Certificate of Analysis for product #53054 DyLight 775-B2 NHS Ester $213.00

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53055 DyLight 775-B3 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 3 sulfonate groups
1mg Product Instructions for product #53055 DyLight 775-B3 NHS Ester MSDS for product #53055 DyLight 775-B3 NHS Ester Certificate of Analysis for product #53055 DyLight 775-B3 NHS Ester $213.00

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85150 DyLight 775-B4 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 4 sulfonate groups
1mg Product Instructions for product #85150 DyLight 775-B4 NHS Ester MSDS for product #85150 DyLight 775-B4 NHS Ester Certificate of Analysis for product #85150 DyLight 775-B4 NHS Ester $213.00

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53064 DyLight 780-B1 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 1 sulfonate group
1mg Product Instructions for product #53064 DyLight 780-B1 NHS Ester MSDS for product #53064 DyLight 780-B1 NHS Ester Certificate of Analysis for product #53064 DyLight 780-B1 NHS Ester $213.00

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53065 DyLight 780-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53065 DyLight 780-B2 NHS Ester MSDS for product #53065 DyLight 780-B2 NHS Ester Certificate of Analysis for product #53065 DyLight 780-B2 NHS Ester $213.00

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53066 DyLight 780-B3 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 3 sulfonate groups
1mg Product Instructions for product #53066 DyLight 780-B3 NHS Ester MSDS for product #53066 DyLight 780-B3 NHS Ester Certificate of Analysis for product #53066 DyLight 780-B3 NHS Ester $213.00

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53067 DyLight 830-B2 NHS Ester
Formulation: NHS ester activated Benzopyrillium with 2 sulfonate groups
1mg Product Instructions for product #53067 DyLight 830-B2 NHS Ester MSDS for product #53067 DyLight 830-B2 NHS Ester Certificate of Analysis for product #53067 DyLight 830-B2 NHS Ester $213.00

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