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DyLight Green-emitting Specialty Dye

Label molecules with green-emitting rhodamine-based dye.

DyLight Green-emitting Specialty Dye

Label peptides, antibodies and other proteins at primary amines with the rhodamine-based, water-soluble Thermo Scientific DyLight Green-emitting Specialty Dye, which is comparable to Alexa Fluor 532.

DyLight Green-emitting Dye is a labeling agent that provides bright fluorescence detection for imaging. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetration in vivo, while more hydrophilic dyes have less nonspecific binding potential. This dye contains 2 sulfonates for increased solubility and an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.


  • NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
  • Water solubility – 2 sulfonate groups increase the solubility of this dye over over less-sulfonated dyes


  • Imaging
  • Antibody labeling
  • Direct immunofluorescence staining
  • Flow cytometry
  • Fluorescence correlation spectroscopy
  • Western blotting
  • Protein microarrays
  • Polymer labeling
  • Peptide labeling
  • Phalloidin labeling for actin staining

Product Details:

DyLight Green-emitting Specialty Dye rhodamine core structure


Thermo Scientific DyLight Green-emitting Specialty Dye Characteristics.
Fluorescent Dye Ex/Em (nm) Solvent 円 Core structure Laser and filter sets
DyLight 530-R2
533/554 PBS 100K Rhodamine, 2 sulfonates 488 laser source
532 flow cytometry laser line
Nd:YAG laser
Argon ion laser
†   Fluorescent dye name appended with abbreviated core structure (e.g., R2: Rhodamine with 2 sulfonates)
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum

Related Resources:

Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide

Related Products:

DyLight 543Q-NHS Ester and 641Q-NHS Ester (complementary fluorescence quenchers)
DyLight 488 Amine-Reactive Dye and Kits

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
46611 DyLight 530-R2 NHS Ester
Formulation: NHS ester activated Rhodamine derivative with 2 sulfonate groups
1mg Product Instructions for product #46611 DyLight 530-R2 NHS Ester MSDS for product #46611 DyLight 530-R2 NHS Ester Certificate of Analysis for product #46611 DyLight 530-R2 NHS Ester $218.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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