Label peptides, antibodies and other proteins at primary amines with benzopyrillium-based Thermo Scientific DyLight Far Red-emitting Specialty Dyes, which are comparable to Alexa Fluor 647 and Cy5.
DyLight Far Red-emitting Dyes are a family of labeling agents that provide bright fluorescent detection for imaging. Dyes can be selected based upon their characteristic excitation and emission properties or relative hydrophilicity and hydrophobicity attributes. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetrating ability in vivo, while more hydrophilic dyes have less nonspecific binding potential. Each dye contains an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.
- Large selection – the largest family of dyes available for far red-emitting fluorescence applications
- NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides, and other amine-containing molecules through amide bond formation
- Multiple solubility options – choose from hydrophilic to hydrophobic dyes to optimize the right dye label for the best performance in a given application
Criteria to consider when choosing a DyLight Far Red-emitting Specialty Dye:
- Excitation and emission wavelengths – choose the best dye to match the excitation and emission capabilities of your instrument.
- Water solubility – choose a dye based on its relative hydrophilicity, which directly correlates to the number of negatively-charged sulfonates it has on its core structure. More hydrophilic dyes are best at maintaining water solubility of a labeled antibody and limiting the nonspecific binding of the conjugate. More hydrophobic dyes often are best at penetrating tissues and cell membranes in vivo, meaning that dyes with fewer sulfonates may work best for some applications.
- DyLight dye selection – the broad selection of red-emitting dyes allows a number of candidate dyes to be tested in a given application for optimal performance.
- Fluorescence imaging
- Confocal microscopy
- Flow cytometry
- Spectral fluorescence imaging
- In vivo imaging
- Fluorescent western blotting
- Protein microarrays
- Antibody labeling
- Peptide labeling
- Fluorescence correlation spectroscopy
- Protein arrays
- Single molecule detection
- Nanoparticle conjugation
- Biotin/streptavidin conjugation
Thermo Scientific DyLight Far Red-emitting Specialty Dye Characteristics.
||Laser and filter sets
||Benzopyrillium, 1 sulfonate
||633 and 647 laser source
633 flow cytometry laser line
Red diode (633) laser
Cy 5 Filter set
||Benzopyrillium, 2 sulfonates
||Benzopyrillium, 3 sulfonates
||Benzopyrillium, 4 sulfonates
|† Fluorescent dye name appended with abbreviated core structure (e.g., B2: Benzopyrillium with 2 sulfonates)
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum
- Luchowski R. et al. (2010) Molecular fluorescence enhancement on fractal-like structures. Applied Spectroscopy. 64, 578-83.
- Chen A. et al. (2008) Assessing the sensitivity of commercially available fluorophores to the intracellular environment. Analytical Chemistry. 80, 7437-44.
- Garcia B. et al. (2007) Antibody microarray analysis of inflammatory mediator release by human leukemia t-cells and human nonâ€“small cell lung cancer cells. Journal of Biomolecular Techniques: JBT. 18, 245.
Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide
DyLight 641Q-NHS Ester, 662Q-NHS Ester and 696Q NHS-Ester (complementary fluorescence quenchers)
DyLight 650 Amine-Reactive Dye and Kits