Thermo Scientific DyLight Fluor Free Acids are non-activated forms of selected DyLight Fluorescent Dyes for use as controls and calibration factors in fluorescence imaging applications. DyLight 488 has excitation and emission peaks at 493 and 518 nm, respectively (±4 nm, in PBS).

These non-activated fluorescent dyes contain the native carboxyl group of the standard dye molecule.

General DyLight Fluorophore Highlights:

• Bright fluorescence—intense emission provides superior sensitivity and requires less conjugate
• Narrow emission spectra—negligible bleed-through between fluorophore channels enables multi-color detection
• Excellent photostability—exceptional resistance to photobleaching enables fluorescence imaging under the most demanding conditions (e.g., structured illumination, 4Pi microscopy)
• Buffer stability—conjugated fluorophores are completely stable at pH 4-9
• Instrument compatibility—excitation and emission spectra correspond with filter sets and laser settings of all popular fluorescence instrumentation

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DyLight™ 747, Free Acid
DyLight™ 800, Free Acid