Label peptides, antibodies and other proteins at primary amines with the coumarin-based, 405 laser-detectable Thermo Scientific DyLight Blue-emitting Specialty Dye.
DyLight Blue-emitting Dye is a labeling agent that provides bright fluorescence detection for imaging and flow cytometry. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetration in vivo, while more hydrophilic dyes have less nonspecific binding potential. This dye contains 1 sulfonate for increased solubility and an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.
- NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
- Water solubility – 1 sulfonate group increases the solubility of this dye over over nonsulfonated dyes
- Molecular imaging
- Flow cytometry
Thermo Scientific DyLight Blue-emitting Specialty Dye Characteristics.
||Laser and filter sets
||Coumarin, 1 sulfonate
||405 laser source
|† Fluorescent dye name appended with abbreviated core structure (e.g., Co1: Coumarin with 1 sulfonate)
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum
Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide
DyLight 425Q-NHS Ester (complementary fluorescence quenchers)
DyLight 405 Amine-Reactive Dye and Kits