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DyLight Blue-emitting Specialty Dye


Label molecules with blue-emitting coumarin-based dye.

DyLight Blue-emitting Specialty Dye

Label peptides, antibodies and other proteins at primary amines with the coumarin-based, 405 laser-detectable Thermo Scientific DyLight Blue-emitting Specialty Dye.

DyLight Blue-emitting Dye is a labeling agent that provides bright fluorescence detection for imaging and flow cytometry. Dyes that contain a greater number of negatively charged sulfonates generally will have greater water solubility than dyes with fewer sulfonates. More hydrophobic dyes often provide better cell penetration in vivo, while more hydrophilic dyes have less nonspecific binding potential. This dye contains 1 sulfonate for increased solubility and an amine-reactive NHS ester for simple modification of antibodies, proteins, peptides or other biomolecules through amide bond formation.

Highlights:

  • NHS ester reactive group – allows immediate labeling of antibodies, proteins, peptides and other amine-containing molecules through amide bond formation
  • Water solubility – 1 sulfonate group increases the solubility of this dye over over nonsulfonated dyes

Applications:

  • Molecular imaging
  • Microscopy
  • Flow cytometry

Product Details:

DyLight Blue-emitting Specialty Dye coumarin core structure

 

Thermo Scientific DyLight Blue-emitting Specialty Dye Characteristics.
Fluorescent Dye Ex/Em (nm) Solvent 円 Core structure Laser and filter sets
DyLight 415-Co1
418/463 Ethanol 34K Coumarin, 1 sulfonate 405 laser source
†   Fluorescent dye name appended with abbreviated core structure (e.g., Co1: Coumarin with 1 sulfonate)
†† Molar extinction coefficient (M-1 cm-1) at the absorption maximum

Related Resources:

Fluorescent labeling technical guide
NHS ester crosslinking chemistry technical guide

Related Products:

DyLight 425Q-NHS Ester (complementary fluorescence quenchers)
DyLight 405 Amine-Reactive Dye and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46609 DyLight 415-Co1 NHS Ester
Formulation: NHS ester activated Coumarin derivative with 1 sulfonate group
1mg Product Instructions for product #46609 DyLight 415-Co1 NHS Ester MSDS for product #46609 DyLight 415-Co1 NHS Ester Certificate of Analysis for product #46609 DyLight 415-Co1 NHS Ester $213.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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