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DyLight 800 Sulfhydryl-Reactive Dye


Label cysteines with this alternative to Alexa Fluor™ 790 and IRDye™ 800 dyes.

DyLight 800 Sulfhydryl-Reactive Dye

Thermo Scientific DyLight 800 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 800 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.

DyLight 800 is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 800 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor™ 790 and IRDye™ 800. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Highlights:

  • High performance – DyLight 800 replaces Alexa Fluor 800 and IRDye 800 for near-infrared staining
  • Specific – maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods – well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure –complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

Product Details:

fluorescent fluorescence Western blot DyLight 680 800 siRNA Two-color infrared Western blot detection using DyLight 680- and DyLight 800-Conjugated Secondary Antibodies. p53 (53 kDa; red) and cyclophilin B (21 kDa; green) levels in response to siRNA expression in A549 cells were detected by Western blot analysis using DyLight 680- (red) and DyLight 800- (green) Conjugated Secondary Antibodies. Beta-catenin (90 kDa, green) was used as the load control. Imaged with the Odyssey™ Infrared Imaging System using the 700 and 800 channels.

DyLight 800 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.

Related Resources:

DyLight 800 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

All Fluorescent Labeling Reagents and Kits
DyLight Fluor Secondary Antibody Conjugates
DyLight Fluor Streptavidin and NeutrAvidin Conjugates
DyLight 800 Fluor, free acid – used as controls and calibrants
Krypton Fluorescent Protein Stains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46621 DyLight 800 Maleimide
Formulation: Maleimide activated proprietary near-infrared fluorescent dye
Sufficient For: 1 use to label a total of 7mg of IgG using typical conditions
1mg Product Instructions for product #46621 DyLight 800 Maleimide MSDS for product #46621 DyLight 800 Maleimide Certificate of Analysis for product #46621 DyLight 800 Maleimide $256.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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