Thermo Scientific DyLight 800-4xPEG Amine-Reactive Dye is a derivative of our high-performance DyLight 800 Dye that can be used to fluorescently label antibodies and other proteins for near-IR detection.
The DyLight 800-4xPEG dye contains 4 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 800-4xPEG Dye can be used as molecular probes for cellular imaging and other fluorescence detection methods. The NIR fluorescence properties of DyLight 800-4xPEG Dye make it especially useful in a variety of biological, chemical, and pharmaceutical applications, including in vivo imaging. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.
- High fluorescence intensity – fluorescence comparable to Alexa Fluor™ 800 and IRDye™ 800
- PEGylated – improves solubility in aqueous solution and aids in cell permeability
- Fluorescence microscopy
- In vivo or ex vivo imaging
- Cell-based assays
- Flow cytometry/fluorescence-activated cell sorting (FACS)
DyLight 800-4xPEG Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.
Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent.
|Thermo Scientific DyLight 800-4xPEG Dye is comparable to other spectrally similar fluors. Infrared Western blot detection of Hsp86 using a rabbit anti-Hsp86 primary antibody and a goat anti-rabbit secondary antibody conjugated to DyLight 800-4xPEG or IRDye 800 (LI-COR Biosciences) dyes.
|Thermo Scientific DyLight 800-4xPEG Dye exhibits rapid biodistribution and tissue clearance. Mice were injected with hydrolyzed DyLight 800-4xPEG or DyLight 800 (non-PEGylated) Dye via the retro-orbital plexus, and NIR in vivo images were captured pre (Pre)- and post-injection at the indicated time points. Data indicate that DyLight 800-4xPEG Dye is more rapidly cleared from the mouse body than the non-PEGylated dye. After the final time point, animals were sacrificed and tissues collected for ex vivo imaging of the kidneys and GI tract to indicate clearance of the dyes via the excretory system. Heat map legends indicate signal intensity (bottom panel).
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DyLight 800 Fluorophore Overview Page
Complete DyLight Fluor Product Guide
DyLight 800 Amine-Reactive Dye and Kits (not PEGylated)
Dye Removal Columns
Pierce Immunostain Enhancer