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DyLight 800-4xPEG Sulfhydryl-Reactive Dye


PEGylated NIR dye for enhanced fluorescence and reduced non-specific binding.

DyLight 800-4xPEG Sulfhydryl-Reactive Dye

Thermo Scientific DyLight 800-4xPEG Sulfhydryl-Reactive Dye is a maleimide-activated derivative of our high-performance DyLight 800 Dye used to fluorescently label cysteine-containing peptides, proteins or other biomolecular probes.

The DyLight 755-4xPEG dye contains 4 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 755-4xPEG Dye can be used as molecular probes for cellular imaging and other fluorescence detection methods. The NIR fluorescence properties of DyLight 755-4xPEG Dye make it especially useful in a variety of biological, chemical, and pharmaceutical applications, including in vivo imaging. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.

Highlights:

  • High fluorescence intensity – fluorescence comparable to Alexa Fluor™ 800 and IRDye™ 800
  • PEGylated – improves solubility in aqueous solution and aids in cell permeability
  • Specific – maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)

Applications:

  • Fluorescence microscopy
  • In vivo or ex vivo imaging
  • Cell-based assays
  • Flow cytometry/fluorescence-activated cell sorting (FACS)

Product Details:

DyLight 800-4xPEG Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.

Related Resources:

DyLight 800 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

DyLight 800 Sulfhydryl-Reactive Dye (not PEGylated)
Dye Removal Columns
Pierce Immunostain Enhancer
Fluorescent Counterstains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46627 DyLight 800-4xPEG Maleimide
Formulation: Maleimide activated proprietary near-infrared fluorescent dye
Sufficient For: 1 use to label a total of 7mg of IgG using typical conditions
1mg Product Instructions for product #46627 DyLight 800-4xPEG Maleimide MSDS for product #46627 DyLight 800-4xPEG Maleimide Certificate of Analysis for product #46627 DyLight 800-4xPEG Maleimide $306.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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