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DyLight 755 Amine-Reactive Dye and Kits


Label amines with a bright alternative to Alexa Fluor™ 750 near-infrared dye.

DyLight 755 Amine-Reactive Dye

Thermo Scientific DyLight 755 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 755 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods.

DyLight 755 is a near-IR fluor that is invisible to the naked eye but increases the staining options when using infrared imaging systems. DyLight 755 has spectral properties that are very similar to other near-IR dyes, including Alexa Fluor™ 750. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 755 Amine-Reactive Dye is available as a stand-alone reagent or as part of two antibody labeling kit sizes.


Highlights:

  • High performance – DyLight 755 replaces Alexa Fluor 755 for near-infrared staining
  • Specific – NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
  • Convenient kit sizes – standard and microscale sizes are offered to match your experimental needs
  • Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

Applications:

  • Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
  • Target protein labeling for in vitro and in vivo fluorescent detection strategies

Product Details:

Fluorescent Western blot using DyLight 755 Infrared Western blot detection of ERK1 using DyLight 755-conjugated secondary antibody. Serial dilutions of A562 cell lysates were analyzed by Western blotting for levels of ERK1 using a mouse anti-ERK1 primary antibody and a goat anti-mouse secondary antibody conjugated to DyLight 755 (using the DyLight 755 Microscale Antibody Labeling Kit). Left lane: Infrared molecular weight marker.

DyLight 755 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 755 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DyLight 755 Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods.The standard size kit contains all necessary components to perform three separate labeling reactions using 1mg of IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100µg of IgG. Both kit sizes include the Amine-Reactive DyLight 755 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Protocol summary for DyLight Antibody Labeling Kits.
Procedure summary for Thermo Scientific DyLight 755 Antibody Labeling Kits. The protocol is simple and provides efficient labeling, exceptional clean-up and excellent antibody recovery.

DyLight Antibody Labeling Kits provide outstanding recovery.
DyLight Antibody Labeling Kits provide outstanding recovery. The percent recovery for DyLight Antibody Labeling Kits is the average for 16 labeling reactions using three different antibodies. The percent recovery for DyLight Microscale Antibody Labeling Kits is the average for 13 labeling reactions using three different antibodies.

Related Resources:

DyLight 755 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

All DyLight Labeling Reagents and Kits
All Fluorescent Labeling Reagents and Kits
DyLight Fluor Secondary Antibody Conjugates
DyLight Fluor Streptavidin and NeutrAvidin Conjugates
Fluorescent Protein Stains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62278 DyLight 755 NHS Ester
Formulation: NHS ester activated proprietary near-infrared fluorescent dye
Sufficient For: 1 use to label a total of 7mg of IgG using typical conditions

Replaces DyLight Fluor 750
1mg Product Instructions for product #62278 DyLight 755 NHS Ester MSDS for product #62278 DyLight 755 NHS Ester Certificate of Analysis for product #62278 DyLight 755 NHS Ester $239.00

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62279 DyLight 755 NHS Ester
Formulation: NHS ester activated proprietary near-infrared fluorescent dye
Sufficient For: 5 uses to label a total of 2mg of IgG using typical conditions

Replaces DyLight Fluor 750
5 x 50µg Product Instructions for product #62279 DyLight 755 NHS Ester MSDS for product #62279 DyLight 755 NHS Ester Certificate of Analysis for product #62279 DyLight 755 NHS Ester $187.00

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84538 DyLight 755 Antibody Labeling Kit
Replaces Part No. 53058
Formulation: Multi-component kit with proprietary near-infrared fluorescent dye
Sufficient For: Labeling and purifying 3 x 1mg (2mg/mL) of IgG or other proteins

Kit Contents:
DyLight Fluor NHS Ester, 3 x 50μg vials
Borate Buffer (0.67M), 1mL
Purification Resin, 5mL
Microcentrifuge Spin Columns, 6 columns
Microcentrifuge Collection Tubes, 12 tubes
3-rxn kit Product Instructions for product #84538 DyLight 755 Antibody Labeling Kit MSDS for product #84538 DyLight 755 Antibody Labeling Kit Certificate of Analysis for product #84538 DyLight 755 Antibody Labeling Kit $328.00

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84539 DyLight 755 Microscale Antibody Labeling Kit
Replaces Part No. 53059
Formulation: Multi-component kit with proprietary near-infrared fluorescent dye
Sufficient For: Labeling and purifying 5 x 100µg (1mg/mL) of IgG or other proteins

Kit Contents:
DyLight Fluor NHS Ester, 5 x 15μg vials
Borate Buffer (0.67M), 1mL
Purification Resin, 5mL
Microcentrifuge Spin Columns, 5 columns
Microcentrifuge Collection Tubes, 10 tubes
5-rxn kit Product Instructions for product #84539 DyLight 755 Microscale Antibody Labeling Kit MSDS for product #84539 DyLight 755 Microscale Antibody Labeling Kit Certificate of Analysis for product #84539 DyLight 755 Microscale Antibody Labeling Kit $328.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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