Thermo Scientific DyLight 755-4xPEG Amine-Reactive Dye is a derivative of our high-performance DyLight 755 Dye that can be used to fluorescently label antibodies and other proteins for near-IR detection.
The DyLight 755-4xPEG dye contains 4 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 755-4xPEG Dye can be used as molecular probes for cellular imaging and other fluorescence detection methods. The NIR fluorescence properties of DyLight 755-4xPEG Dye make it especially useful in a variety of biological, chemical, and pharmaceutical applications, including in vivo imaging. The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.
- High fluorescence intensity – fluorescence comparable to Alexa Fluor™ 750
- PEGylated – improves solubility in aqueous solution and aids in cell permeability
- Fluorescence microscopy
- In vivo or ex vivo imaging
- Cell-based assays
- Flow cytometry/fluorescence-activated cell sorting (FACS)
DyLight 755-4xPEG Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.
Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent.
|Thermo Scientific DyLight 755-4xPEG Dye is comparable to other spectrally similar fluors. Infrared Western blot detection of α-tubulin using a mouse anti-α-tubulin primary antibody and a goat anti-mouse secondary antibody conjugated to DyLight 755-4xPEG, Alexa Fluor 750 (Life Technologies Corp.) or CF 750 (Biotium Inc.) dyes.
|Thermo Scientific DyLight 755-4xPEG Dye exhibits rapid biodistribution and tissue clearance. Mice were injected with hydrolyzed DyLight 755-4xPEG Dye or DyLight 755 (non-PEGylated) Dye via the retro-orbital plexus, and NIR in vivo images were captured pre (Pre)- and post-injection at the indicated time points (top images). Data indicate that both dyes are rapidly distributed throughout the mouse body, but the PEGylated dye is more rapidly cleared than the non-PEGylated dye. After the final time point, animals were sacrificed and tissues collected for ex vivo imaging of the kidneys and GI tract to indicate clearance of the dyes via the excretory system. Heat map legends indicate signal intensity (bottom panel).
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DyLight 755 Fluorophore Overview Page
Complete DyLight Fluor Product Guide
DyLight 755 Amine-Reactive Dye and Kits (not PEGylated)
Dye Removal Columns
Pierce Immunostain Enhancer