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DyLight 680 Sulfhydryl-Reactive Dye


Label cysteines with a bright alternative to Alexa Fluor™ 680 and Cy5.5™ dyes.

DyLight 680 Sulfhydryl-Reactive Dye

Thermo Scientific DyLight 680 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 680 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.

DyLight 680 produces near-infrared (IR) fluorescence that replaces other near-IR dyes, including Cy5.5™ dye and Alexa Fluor™ 680, and is ideal for multi-color applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Highlights:

  • High performance – DyLight 680 fluoresces brighter than Alexa Fluor 680 and Cy5.5 dyes
  • Specific – maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods – well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure –complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

Product Details:

Fluorescent detection of cytokeratin in A549 cells with DyLight 680
Immunofluorescence microscopy using DyLight 680 dye. Cytokeratin 18 (pseudo-colored yellow) and lamin A (blue) in A549 cells were fluorescently labeled with their respective primary antibodies and DyLight 680-Conjugated Goat Anti-Rabbit or DyLight 405-Conjugated Goat Anti-Mouse Secondary Antibodies, respectively.

DyLight 680 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.

Related Resources:

DyLight 680 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

All Fluorescent Labeling Reagents and Kits
DyLight Fluor Secondary Antibody Conjugates
DyLight Fluor Streptavidin and NeutrAvidin Conjugates
Krypton Fluorescent Protein Stains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46618 DyLight 680 Maleimide
Formulation: Maleimide activated proprietary near-infrared fluorescent dye
Sufficient For: 1 use to label a total of 8mg of IgG using typical conditions
1mg Product Instructions for product #46618 DyLight 680 Maleimide MSDS for product #46618 DyLight 680 Maleimide Certificate of Analysis for product #46618 DyLight 680 Maleimide $256.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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