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DyLight 650-4xPEG Sulfhydryl-Reactive Dye


PEGylated far-red dye for enhanced fluorescence and reduced non-specific binding.

DyLight 650-4xPEG Sulfhydryl-Reactive Dye

Thermo Scientific DyLight 650-4xPEG Sulfhydryl-Reactive Dye is a maleimide-activated derivative of our high-performance DyLight 650 Dye used to fluorescently label cysteine-containing peptides, proteins or other biomolecular probes.

The DyLight 650-4xPEG Dye contains 4 polyethylene glycol (PEG) chains that are non-toxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 650-4xPEG Dye can be used as molecular probes for cellular and in vivo imaging, flow cytometry, IHC and other fluorescence detection methods.The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.

Highlights:

  • High fluorescence intensity – significantly brighter fluorescence than Alexa Fluor™ 647
  • PEGylated – improves solubility in aqueous solution and aids in cell permeability
  • Specific – maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)

Applications:

  • Fluorescence microscopy
  • In vivo or ex vivo imaging
  • Cell-based assays
  • Flow cytometry/fluorescence-activated cell sorting (FACS)

Product Details:

DyLight 650-4xPEG Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.

Related Resources:

DyLight 650 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

DyLight 650 Sulfhydryl-Reactive Dye (not PEGylated)
Dye Removal Columns
Pierce Immunostain Enhancer
Fluorescent Counterstains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62294 DyLight 650-4xPEG Maleimide
Formulation: Maleimide activated proprietary far-red fluorescent dye
Sufficient For: 1 use to label a total of 7mg of IgG using typical conditions
1mg Product Instructions for product #62294 DyLight 650-4xPEG Maleimide MSDS for product #62294 DyLight 650-4xPEG Maleimide Certificate of Analysis for product #62294 DyLight 650-4xPEG Maleimide $306.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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