Thermo Scientific DyLight 550-2xPEG Amine-Reactive Dye is a derivative of our high-performance DyLight 550 Dye that can be used to fluorescently label antibodies and other proteins.
The DyLight 550-2xPEG dye contains 2 polyethylene glycol (PEG) chains that are non-cytotoxic, enhance fluorescence and reduce nonspecific binding of conjugates made with them. Conjugates made with DyLight 550-2xPEG Dye can be used as molecular probes for cellular imaging, flow cytometry and other fluorescence detection methods.The PEG chains also improve solubility of the dyes and labeled molecules in aqueous solution, aid in cell permeability and improve tissue retention.
- High fluorescence intensity – significantly brighter fluorescence than Alexa Fluor™ 555
- PEGylated – improves solubility in aqueous solution and aids in cell permeability
- Fluorescence microscopy
- In vivo or ex vivo imaging
- Cell-based assays
- Flow cytometry/fluorescence-activated cell sorting (FACS)
DyLight 550-2xPEG Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.
Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to the labeling reagent.
|Thermo Scientific DyLight 550-2xPEG Dye is brighter than other spectrally similar fluors. The endoplasmic reticulum in U20S osteosarcoma cells was fluorescently labeled with mouse anti-PDI primary antibody and goat anti-mouse secondary antibody conjugated to DyLight 550-2xPEG, Alexa Fluor 555 (Life Technologies Corp.) or CF 555 (Biotium Inc.) dyes. All images were captured using the same exposure time and contrast and brightness settings.
|Thermo Scientific DyLight 550-2xPEG Dye is great for IHC. Paraffin-embedded normal colon tissue was fluorescently probed for the EGF receptor with rabbit anti-EGFR antibody (Thermo Scientific EGFR antibody, PA1-1110) and DyLight 550-2xPEG Dye-conjugated goat anti-rabbit secondary antibody. Nuclei were also counterstained with Hoechst 33342 dye.
|Thermo Scientific DyLight 550-2xPEG Dye is compatible with flow cytometers. The LFA1 cell surface adhesion glycoprotein in Jurkat cells was fluorescently labeled with mouse anti-LFA1 primary antibody and DyLight 550-2xPEG Dye-conjugated goat anti-mouse secondary antibody. The stained cells were then counted using the BD™ Accuri™ C6 Flow Cytometer.
DyLight 550 Fluorophore Overview Page
Complete DyLight Fluor Product Guide
DyLight 550 Amine-Reactive Dye and Kits (not PEGylated)
Dye Removal Columns
Pierce Immunostain Enhancer