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DyLight 488 Amine-Reactive Dye and Kits


Label proteins with this alternative to Alexa Fluor™ 488, Cy2™ and FITC dyes.

DyLight 488 Amine-Reactive Dye

Thermo Scientific DyLight 488 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 488 for fluorescent labeling of antibodies and other proteins to be used as molecular probes for cellular imaging and other fluorescence detection methods.

DyLight 488 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Cy2™, Alexa™ Fluor 488, FITC and LI-COR™ dyes in many applications. The high water solubility of DyLight Fluors allows a high dye-to-protein ratio to be achieved without causing precipitation of the conjugates. DyLight 488 Amine-Reactive Dye is available as a stand-alone reagent or as part of two antibody labeling kit sizes.

Highlights:

  • High performance – DyLight 488 is comparable to Alexa Fluor 488 and brighter than FITC and Cy2
  • Specific – NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
  • Convenient kit sizes – standard and microscale sizes are offered to match your experimental needs
  • Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

Applications:

  • Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
  • Target protein labeling for in vitro and in vivo fluorescent detection strategies

Product Details:

Fluorescence detection of DyLight 488 dye

Immunofluorescence microscopy using DyLight 488 dye. Phosphorylated EGFR (green) in A431 cells was labeled with a specific primary antibody and DyLight 488-Conjugated Goat Anti-Mouse Secondary Antibody. The nuclei were stained with Hoechst dye.

DyLight 488 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require the dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 488 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DyLight 488 Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods.The standard size kit contains all necessary components to perform three separate labeling reactions using 1mg of IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100µg of IgG. Both kit sizes include the Amine-Reactive DyLight 488 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Protocol summary for DyLight Antibody Labeling Kits.
Procedure summary for DyLight 488 Antibody Labeling Kits. The protocol is simple and provides efficient labeling, exceptional clean-up and excellent antibody recovery.

DyLight Antibody Labeling Kits provide outstanding recovery.
DyLight Antibody 488 Labeling Kits provide outstanding recovery. The percent recovery for DyLight Antibody Labeling Kits is the average for 16 labeling reactions using three different antibodies. The percent recovery for DyLight Microscale Antibody Labeling Kits is the average for 13 labeling reactions using three different antibodies.

Related Resources:

DyLight 488 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

All DyLight Labeling Reagents and Kits
All Fluorescent Labeling Reagents and Kits
DyLight Fluor Secondary Antibody Conjugates
DyLight Fluor Streptavidin and NeutrAvidin Conjugates
DyLight 488 Azide-Reactive Dye – phosphine-activated fluorescent dye for Staudinger ligation
DyLight 488 Fluor, free acid – used as controls and calibrants
Fluorescent Protein Stains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46402 DyLight 488 NHS Ester
Formulation: NHS ester activated proprietary green fluorescent dye
Sufficient For: 1 use to label a total of 7mg of IgG using typical conditions
1mg Product Instructions for product #46402 DyLight 488 NHS Ester MSDS for product #46402 DyLight 488 NHS Ester Certificate of Analysis for product #46402 DyLight 488 NHS Ester $239.00

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46403 DyLight 488 NHS Ester
Formulation: NHS ester activated proprietary green fluorescent dye
Sufficient For: 5 uses to label a total of 2mg of IgG using typical conditions
5 x 50µg Product Instructions for product #46403 DyLight 488 NHS Ester MSDS for product #46403 DyLight 488 NHS Ester Certificate of Analysis for product #46403 DyLight 488 NHS Ester $187.00

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46402B DyLight 488 NHS Ester
Formulation: NHS ester activated proprietary green fluorescent dye
Custom MSDS for product #46402B DyLight 488 NHS Ester Certificate of Analysis for product #46402B DyLight 488 NHS Ester
53024 DyLight 488 Antibody Labeling Kit
Formulation: Multi-component kit with proprietary green fluorescent dye
Sufficient For: Labeling and purifying 3 x 1mg (2mg/mL) of IgG or other proteins

Kit Contents:
DyLight Fluor NHS Ester, 3 x 50μg vials
Borate Buffer (0.67M), 1mL
Purification Resin, 5mL
Microcentrifuge Spin Columns, 6 columns
Microcentrifuge Collection Tubes, 12 tubes
3-rxn kit Product Instructions for product #53024 DyLight 488 Antibody Labeling Kit MSDS for product #53024 DyLight 488 Antibody Labeling Kit Certificate of Analysis for product #53024 DyLight 488 Antibody Labeling Kit $350.00

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53025 DyLight 488 Microscale Antibody Labeling Kit
Formulation: Multi-component kit with proprietary green fluorescent dye
Sufficient For: Labeling and purifying 5 x 100µg (1mg/mL) of IgG or other proteins

Kit Contents:
DyLight Fluor NHS Ester, 5 x 15μg vials
Borate Buffer (0.67M), 1mL
Purification Resin, 5mL
Microcentrifuge Spin Columns, 5 columns
Microcentrifuge Collection Tubes, 10 tubes
5-rxn kit Product Instructions for product #53025 DyLight 488 Microscale Antibody Labeling Kit MSDS for product #53025 DyLight 488 Microscale Antibody Labeling Kit Certificate of Analysis for product #53025 DyLight 488 Microscale Antibody Labeling Kit $328.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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