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DyLight 405 Sulfhydryl-Reactive Dye


A bright alternative to Alexa Fluor™ 405, Cascade™ Blue and AMCA dyes.

DyLight 405 Sulfhydryl-Reactive Dye

Thermo Scientific DyLight 405 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 405 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.

DyLight 405 has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than Alexa Fluor™ 405, Cascade™ Blue and AMCA dyes in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.

Highlights:

  • High performance – DyLight 405 shows brighter fluorescence than Alexa Fluor 405, Cascade Blue and AMCA
  • Specific – maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
  • Efficient labeling methods – well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
  • Optimized antibody labeling procedure –complete protocol for IgG reduction and labeling and calculating the labeling efficiency

Applications:

  • Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
  • Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies

Product Details:

Fluorescence detection of DyLight 405 dye
Immunofluorescence microscopy using DyLight 405 dye. Vimentin (red) and lamin B1 (blue) in normal human colon tissue were fluorescently labeled with specific primary antibodies and DyLight 405-Conjugated Goat Anti-Mouse or DyLight 549-Conjugated Goat Anti-Rabbit Secondary Antibodies, respectively. Cell nuclei were counterstained green.

DyLight 405 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.

Related Resources:

DyLight 405 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

All Fluorescent Labeling Reagents and Kits
DyLight Fluor Secondary Antibody Conjugates
DyLight Fluor Streptavidin and NeutrAvidin Conjugates
Krypton Fluorescent Protein Stains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46600 DyLight 405 Maleimide
Formulation: Maleimide activated proprietary blue fluorescent dye
Sufficient For: 1 use to label a total of 9mg of IgG using typical conditions
1mg Product Instructions for product #46600 DyLight 405 Maleimide MSDS for product #46600 DyLight 405 Maleimide Certificate of Analysis for product #46600 DyLight 405 Maleimide $256.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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