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DyLight 350 Amine-Reactive Dye and Kits


Label proteins with a bright alternative to Alexa Fluor™ 350 and AMCA dyes.

DyLight 350 Amine-Reactive Dye

Thermo Scientific DyLight 350 Amine-Reactive Dye is an NHS ester-activated derivative of high-performance DyLight 350 used to fluorescently label antibodies and other proteins that are then used as molecular probes for cellular imaging and other fluorescence detection methods.

DyLight 350 is a derivative of aminomethylcoumarine acetate (AMCA) and has a higher fluorescence intensity than Alexa Fluor™ 350 and AMCA in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates. DyLight 350 Amine-Reactive Dye is available as a stand-alone reagent or as part of two antibody labeling kit sizes.

Highlights:

  • High performance – DyLight 350 shows brighter fluorescence than Alexa Fluor 350 and AMCA
  • Specific – NHS ester-activated dye labels proteins and other molecules at primary amines (-NH2)
  • Convenient kit sizes – standard and microscale sizes are offered to match your experimental needs
  • Optimized procedure – following the standard protocol results in antibodies with excellent dye:protein ratios and recovery rates for optimum activity and fluorescence labeling

Applications:

  • Primary antibody labeling for immunofluorescence microscopy, immunohistochemistry (IHC), Western blotting or ELISA assay
  • Target protein labeling for in vitro and in vivo fluorescent detection strategies

Product Details:

Fluorescence detection of DyLight 350 dye
Immunofluorescence microscopy using DyLight 350 dye. Alpha-tubulin (blue) and Lamin B1 (green) in A549 cells were fluorescently labeled with their respective primary antibodies and DyLight 350-Conjugated Highly Cross-Adsorbed Goat Anti-Mouse or DyLight 488-Conjugated Goat Anti-Rabbit Secondary Antibodies, respectively.

DyLight 350 Amine-Reactive Dye is activated with an N-hydroxysuccinimide (NHS) ester moiety to react with exposed N-terminal α-amino groups or the ε-amino groups of lysine residues to form stable amide bonds. Learn more about NHS ester chemistry.

Typical labeling reactions require DyLight 350 Amine-Reactive Dye to first be dissolved in anhydrous dimethyl formamide (DMF) or another suitable organic solvent before adding a specific molar amount of dye to an amine-free buffer containing the protein to be labeled. However, the high solubility of DyLight Fluors permits protein solutions to be added directly to specific amounts of the labeling reagent. This feature allows DyLight 350 Amine-Reactive Dye to be provided in multiple formats with flexible protocols to achieve efficient degrees of labeling.

We also offer Standard and Microscale DyLight 350 Antibody Labeling Kits for fast and efficient fluorescent labeling of antibodies for use in fluorescence methods.The standard size kit contains all necessary components to perform three separate labeling reactions using 1mg of IgG or similar quantities of other proteins. The microscale kit contains all of the necessary components to perform five separate labeling reactions using 100µg of IgG. Both kit sizes include the Amine-Reactive DyLight 350 NHS-ester in convenient single-use vials as well as purification resin and spin columns for the preparation of ready-to-use conjugate.

Protocol summary for DyLight Antibody Labeling Kits.
Procedure summary for DyLight 350 Antibody Labeling Kits. The protocol is simple and provides efficient labeling, exceptional clean-up and excellent antibody recovery.

DyLight Antibody Labeling Kits provide outstanding recovery.
DyLight Antibody Labeling Kits provide outstanding recovery. The percent recovery for DyLight Antibody Labeling Kits is the average for 16 labeling reactions using three different antibodies. The percent recovery for DyLight Microscale Antibody Labeling Kits is the average for 13 labeling reactions using three different antibodies.

Related Resources:

DyLight 350 Fluorophore Overview Page
Complete DyLight Fluor Product Guide

Related Products:

All Fluorescent Labeling Reagents and Kits
DyLight Fluor Secondary Antibody Conjugates
DyLight Fluor Streptavidin and NeutrAvidin Conjugates
Krypton Fluorescent Protein Stains


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
46426 DyLight 350 NHS Ester
Formulation: NHS ester activated proprietary blue fluorescent dye
Sufficient For: 1 use to label a total of 9mg of IgG using typical conditions
1mg Product Instructions for product #46426 DyLight 350 NHS Ester MSDS for product #46426 DyLight 350 NHS Ester Certificate of Analysis for product #46426 DyLight 350 NHS Ester $201.00

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46427 Dylight 350 NHS Ester
Formulation: NHS ester activated proprietary blue fluorescent dye
Sufficient For: 5 uses to label a total of 3mg of IgG using typical conditions
5 x 65µg Product Instructions for product #46427 Dylight 350 NHS Ester MSDS for product #46427 Dylight 350 NHS Ester Certificate of Analysis for product #46427 Dylight 350 NHS Ester $187.00

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62275 DyLight 350 Antibody Labeling Kit
Formulation: Multi-component kit with proprietary blue fluorescent dye
Sufficient For: Labeling and purifying 3 x 1mg (2mg/mL) of IgG or other proteins

Kit Contents:
DyLight Fluor NHS Ester, 3 x 65μg vials
Borate Buffer (0.67M), 1mL
Purification Resin, 5mL
Microcentrifuge Spin Columns, 6 columns
Microcentrifuge Collection Tubes, 12 tubes
3-rxn kit Product Instructions for product #62275 DyLight 350 Antibody Labeling Kit MSDS for product #62275 DyLight 350 Antibody Labeling Kit Certificate of Analysis for product #62275 DyLight 350 Antibody Labeling Kit $328.00

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62276 DyLight 350 Microscale Antibody Labeling Kit
Formulation: Multi-component kit with proprietary blue fluorescent dye
Sufficient For: Labeling and purifying 5 x 100µg (1mg/mL) of IgG or other proteins

Kit Contents:
DyLight Fluor NHS Ester, 5 x 15μg vials
Borate Buffer (0.67M), 1mL
Purification Resin, 5mL
Microcentrifuge Spin Columns, 5 columns
Microcentrifuge Collection Tubes, 10 tubes
5-rxn kit Product Instructions for product #62276 DyLight 350 Microscale Antibody Labeling Kit MSDS for product #62276 DyLight 350 Microscale Antibody Labeling Kit Certificate of Analysis for product #62276 DyLight 350 Microscale Antibody Labeling Kit $328.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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