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Dye Removal Columns

Developed especially for fluorescent antibody labeling clean-up.

Pierce Dye Removal Columns

Thermo Scientific Pierce Dye Removal Columns effectively bind to unconjugated fluorescent dye molecules from protein solutions to rapidly purify fluorescent conjugated antibodies and other proteins after labeling reactions.

These Fluorescent Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions. Removing excess dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The dye removal resin in this kit is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated dye. Using the appropriate amount of resin and buffer conditions, almost any fluorescent dye can be removed with this kit.


  • Fast – removes free, unconjugated dye from labeled protein solutions in less than a minute
  • Specific – purification resin provides outstanding conjugate recovery (75 to 95%)
  • Customizable – separate resin and microcentrifuge columns allow optimization for different dyes and concentrations of sample
  • Sample-friendly – processing results in minimal sample dilution

Product Details:

This product has been used successfully to remove excess, unconjugated fluorescein, rhodamine and DyLight Dyes from antibodies following labeling reactions. Samples with dyes in the green spectrum, such as Dylight 549 and rhodamine, might require double processing because they require a high molar excesses for labeling. When using fluorescent dyes other than those described in the product instructions, the ratio of resin to protein/dye mixture will require optimization.

Conjugate recovery rates of fluorescent dye removal columns
Excellent protein conjugate recoveries with Thermo Scientific Pierce Dye Removal Columns. Goat anti-mouse IgG (GAM, n=12), goat anti-rabbit IgG (GAR, n=8) and streptavidin (SA, n=4) proteins were labeled with FITC, NHS-Fluorescein and NHS-Rhodamine and then purified from unreacted dye using Pierce Dye Removal Columns.

Related Resources:

Dye Removal Columns FAQ
Review of Fluorescent Probes
Tech Tip #31 – Calculate dye:protein (F/P) molar ratios

Related Products:

DyLight Fluor Labeling Reagents and Kits
Fluorescent Labeling – Top-level menu of all reagents

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22858 Dye Removal Columns
Formulation: Multi-component kit
Sufficient For: 10 purifications, each with 0.25mL of a dye-labeled protein sample

Kit Contents:
Purification Resin, 5mL
Microcentrifuge Spin Columns, 10 columns
Microcentrifuge Collection Tubes, 20 tubes
10-column kit Product Instructions for product #22858 Dye Removal Columns MSDS for product #22858 Dye Removal Columns Certificate of Analysis for product #22858 Dye Removal Columns $116.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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