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DTSSP (3,3ยด-Dithiobis[sulfosuccinimidylpropionate])


The water-soluble form of Lomant's Reagent (DSP).

Pierce DTSSP

Thermo Scientific Pierce DTSSP is a popular water-soluble crosslinker that contains amine-reactive NHS-ester ends around an 8-atom spacer arm, whose central disulfide bond can be cleaved with reducing agents.

DTSSP is 3,3´-Dithiobis(sulfosuccinimidylpropionate), which contains an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester at each end of an 8-carbon spacer arm. Sulfo-NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysulfosuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for sulfo-NHS-ester crosslinking reagents. DSS, the non-sulfonated analog of DTSSP is also available for applications that require a membrane-permeable crosslinker.

Highlights:

  • Reactive groups: sulfo-NHS ester (both ends)
  • Reactive towards: amino groups (primary amines)
  • Sulfo-NHS ester reacts rapidly with any primary amine-containing molecule
  • Disulfide bond in the spacer arm is readily cleaved by 10-50mM DTT or TCEP at pH 8.5
  • Spacer arm also easily cleaved with reducing SDS-PAGE sample loading
  • Cleavable crosslinker allows separation of crosslinked products
  • Water-soluble; compare with DSP
  • Membrane-impermeable, allowing for cell surface labeling

Applications:

  • Chemical crosslinking of cell surface proteins prior to cell lysis and immunoprecipitation
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces

Product Details:

3,3'-Dithiobis(sulfosuccinimidylpropionate); DTSSP
 
Chemical structure of DTSSP crosslinking reagent.

 

Properties of DTSSP.
Alternative names Sulfo-DSP
Molecular formula C14H14O14N2S4Na2
Molecular weight 608.51
Spacer arm length 12.0 Å (8 atoms)
Storage conditions 4°C, protect from moisture, use only fresh solutions
Reactive groups Sulfo-NHS esters, react with primary amines at pH 7.0-9.0

DTSSP Specifications:

We manufacture DTSSP to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of DTSSP is tested to meet the following minimum specifications.

  • Solubility: >6mg/mL in DI water
  • Identity: IR scan shows only peaks characteristic of the structure and functional groups of DTSSP
  • Purity:> 80% by quantitative NMR (the highest standard for crosslinker purity)

References:

  1. Back, J. W., et al. (2002). Protein Sci. 11, 2471-2478.
  2. Bennett, K.L., et al. (2000). Protein Sci. 9, 1503-1518.
  3. Cheng, S.K., et al. (2004) PloS Biology.2, e30.
  4. Paine-Saunders, S., et al. (2002). J. Biol. Chem.277, 2089-2096.
  5. Liliana Schaefer, L., et al. (2005). J. Clin. Invest.115, 2223-2233.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)
BS3 (non-cleavable)
DSP (membrane permeable)
Bioconjugate Techniques (book)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
21578 DTSSP
Formulation: 3,3'-Dithiobis(sulfosuccinimidylpropionate); powder solid
Sufficient For: 82mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21578 DTSSP MSDS for product #21578 DTSSP Certificate of Analysis for product #21578 DTSSP $105.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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