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A mid-length, maleimide crosslinker with an internal disulfide bond for reversible sulfhydryl conjugation.

Pierce DTME

Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.


  • Reactive groups: maleimide (both ends)
  • Reactive towards: sulfhydryl groups
  • Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
  • Water-insoluble – dissolve first in DMF or DMSO, then add to aqueous reaction buffers
  • Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent

Product Details:

Dithiobismaleimidoethane; DTME
Chemical structure of DTME crosslinking reagent.


  1. Chen, L.L., et al. (1991). Production of multimeric forms of CD4 through a sugar-based crosslinking strategh. J. Biol. Chem. 266(27), 18237-18243.
  2. Han, J.C. and Han, G.Y. (1994). A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothretiol. Anal. Biochem. 220, 5-10.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
22335 DTME
Formulation: Dithiobismaleimidoethane; solid
Sufficient For: 800mL of typical 1X (0.2mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #22335 DTME MSDS for product #22335 DTME Certificate of Analysis for product #22335 DTME $247.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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