Thermo Scientific Pierce DTME is a mid-length, maleimide crosslinker for reversible covalent conjugation between sulfhydryl groups (e.g., protein or peptide cysteines) by reduction of the disulfide bond in the center of the spacer arm.
- Reactive groups: maleimide (both ends)
- Reactive towards: sulfhydryl groups
- Long (13.3A), cleavable, sulfhydryl-to-sulfhydryl crosslinker, composed of maleimide groups and 11-atom disulfide spacer arm
- Water-insoluble – dissolve first in DMF or DMSO, then add to aqueous reaction buffers
- Cleavable by reduction of disulfide spacer arm with DTT, TCEP or other reducing agent
|Chemical structure of DTME crosslinking reagent.
- Chen, L.L., et al. (1991). Production of multimeric forms of CD4 through a sugar-based crosslinking strategh. J. Biol. Chem. 266(27), 18237-18243.
- Han, J.C. and Han, G.Y. (1994). A procedure for quantitative determination of tris(2-carboxyethyl)phosphine, an odorless reducing agent more stable and effective than dithiothretiol. Anal. Biochem. 220, 5-10.
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Overview of crosslinking
Chemistry of crosslinking
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