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Premium Grade DSP

Thoroughly tested, highly characterized and fully documented batches of reagent.

Pierce Premium Grade DSP Crosslinker

Thermo Scientific Pierce Premium Grade DSP is our highest quality formulation of this popular amine-reactive, cleavable crosslinker, specially characterized for applications where product integrity and risk minimization are paramount.

Compared to the standard grade product, Premium Grade DSP provides more clearly defined quality and product support by including (a) increased analytical testing and product characterization, (b) greater batch-specific information and quality assurance review, (c) extensive lot sample retention and (d) change control notification. DSP is dithiobis(succinimidyl propionate), an amine-reactive crosslinker having NHS-ester reactive ends and a cleavable disulfide bond in its spacer arm. DSP contains an amine-reactive N-hydroxysuccinimide (NHS) ester at each end of an 8-carbon spacer arm. NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxy-succinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents.


  • DSP – a thiol-cleavable, homobifunctional and amine-reactive crosslinking agent
  • High quality – identity and purity confirmed by several tests, including quantitative NMR
  • Product integrity – enhanced level of testing and characterization compared to standard grade
  • Lot retention – ample supply of past lots retained to ensure future process testing
  • Change management – Change Control Notification (CCN) service
  • Consistent manufacture – batch-specific manufacturing documentation review


  • Chemical crosslinking of intracellular proteins prior to cell lysis and immunoprecipitation
  • “Fix” protein interactions to allow identification of weak or transient protein interactions
  • Fix tissues for immunostaining without damaging RNA
  • Protein crosslinking to create bioconjugates via single-step reactions
  • Immobilize proteins onto amine-coated surfaces

Product Details:

Dithiobis(succinimidylpropionate); DSP
Chemical structure of DSP crosslinking reagent. For more complete information about the chemical properties and uses of this reagent, see the product page for standard grade DSP.


Specifications of Premium Grade DSP. We manufacture crosslinking reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications.
Test Premium Grade Specification Standard Grade
Purity Greater than 95% by quantitative NMR using an internal standard (the highest standard for crosslinker purity) Yes
Visual White to off-white powder, free of foreign material Yes
Identity IR scan shows only peaks characteristic of DSP Yes
Mass identity Molecular weight of 404 m/z by mass spectrometry
Water content Karl Fischer titration, ≤ 2%
Trace metals All metals, including lead, ≤ 50 ppm
Elemental analysis Reported values for C, H, N, O, and S

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Premium Grade Reagents – complete menu
DSP (standard grade)
Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
PG82081 Premium Grade DSP
Formulation: Dithiobis(succinimidylpropionate); powder solid
Sufficient For: 2.47L of typical 1X (1mM) reaction solution
1g Product Instructions for product #PG82081 Premium Grade DSP MSDS for product #PG82081 Premium Grade DSP Certificate of Analysis for product #PG82081 Premium Grade DSP $256.00

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PG82082 Premium Grade DSP
Formulation: Dithiobis(succinimidylpropionate); powder solid
Sufficient For: 24.7L of typical 1X (1mM) reaction solution
10g Product Instructions for product #PG82082 Premium Grade DSP MSDS for product #PG82082 Premium Grade DSP Certificate of Analysis for product #PG82082 Premium Grade DSP $1,794.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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