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DRAQ5 Fluorescent Probe


A red to infrared DNA content stain for fluorescence microscopy and cellular imaging.

DRAQ5 far-red fluorescent DNA stain

The Thermo Scientific DRAQ5™ Fluorescent Probe is a far-red DNA stain for live or fixed cells and easy multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins.

DRAQ5™ Fluorescent Probe is a far-red DNA stain for fluorescent cellular imaging applications with live or fixed cells. Because of its far-red excitation and emission, the DRAQ5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ5 Fluorescent Probe is compatible with many existing protocols across a wide range of instrumentation platforms.

Highlights:

  • DRAQ5 dye – a red-to-infrared fluorescent stain specific for DNA (nuclei and DNA quantitation)
  • Convenient – provided as easy-to-use DRAQ5 dye solutions (5mM)
  • Cellular imaging – lipophilic and membrane permeable dye is effective for live-cell and fixed-cell staining and quantitation of DNA content
  • Counterstain – ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS)

Applications:

  • Flow cytometry
  • Flow and laser scanning cytometry
  • Live cell imaging
  • Cell based assays

Properties of DRAQ5 Fluorescent Dye:

Structure Proprietary
Alternative names Anthraquinone compound
Chemical name(s) 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione
Molecular formula Not available
Molecular weight 412.54
Excitation wavelength 647nm line optimal (Exλmax = 646nm)
Emission wavelength > 665nm to infra-red > 800nm
(Emλmax = 681nm / 697nm intercalated with dsDNA)
Extinction coefficient Not available
CAS # Not available
Purity > 99%
Solubility Soluble at working concentrations in water and typical buffers
Storage Store at 4°C or room temperature, protected from light
Reactive groups None; binds DNA A-T minor groove, mimicking histone 2b distribution, resulting in 16nm red-shift

Product Details:

DRAQ5 Dye, a red fluorescent DNA stain
DRAQ5 Dye is a red fluorescent DNA stain. HepG2 cells stained with Thermo Scientific DRAQ5 Fluorescent Probe.

Fluorescence cell-based assays, such as flow cytometry, in-cell ELISA, fluorescence microscopy and high-content imaging require a fluorescent label to identify individual cells. When using multiple fluorescent probes to detect different cellular targets or activities, each probe must have a fluorescent spectrum different than the other probes. The blue-fluorescent DNA-binding probes, Hoechst and DAPI are frequently used; however, these probes cannot be used when UV illumination is unavailable or other blue-emitting fluorescent probes are used. Therefore, nuclear probes that emit in a color other than blue are useful for cell identification and counting, and for determining nuclear morphology and DNA content.

Use DRAQ5 dye alongside blue fluorescent probes.
DRAQ5 Dye provides DNA counterstaining in experiments involving blue fluorescent probes. Glutathione (GSH) is rapidly depleted in A549 cells upon 300μM menadione treatment for 30 minutes at 37ºC, resulting in decreased staining by blue-fluorescent monochlorobimane (MBCL) and monobromobimane (MBBR). Fluorescence intensity in separate blue and red channels allows quantitation. Cell images were acquired on the Thermo Scientific ArrayScan HCS Reader with a 20X objective lens.

DRAQ5 Fluorescent Probe emits in the far-red region, is lipophilic and crosses cell and nuclear membranes in live and fixed cells and tissues for rapid DNA staining. This stain is water-soluble, supplied ready to use and does not require RNase, cell lysis, or a washing step, making it compatible with automation. Because DNA staining is stoichiometric, the DRAQ5 Fluorescent Probe can be used for DNA content analysis in cell proliferation studies. DRAQ5 Fluorescent Probe is usually added as the last stain in a labeling procedure because no washing is required. Alternatively, add this probe in assay medium for a live cell assay.

References:

  1. Edward, R. (2009). Use of DNA-specific anthraquinone dyes to directly reveal cytoplasmic and nuclear boundaries in live and fixed cells. Mol Cells 27:391-6.
  2. Martin, R.M., et al. (2005). DNA labeling in living cells. Cytometry 67A:45-52.
  3. Smith, P.J., et al. (1999). A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J Immunol Methods 229(1-2):131-9.
  4. Smith, P.J., et al. (2000). Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry 40(4):280-91.
  5. Swerts, K., et al. (2007). DRAQ5: Improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies. Clin Chim Acta 379:154-157.
  6. Wiltshire, M., et al. (2000). A novel deep red/low infrared fluorescent flow cytometric probe, DRAQ5NO, for the discrimination of intact nucleated cells in apoptotic cell populations. Cytometry 39(3):217-23.
  7. Yuan, C.M., et al. (2004). DRAQ5-based DNA content analysis of hematolymphoid cell subpopulations discriminated by surface antigens and light scatter properties. Cytometry 58:4752.

Related Resources:

Immunohistochemistry (IHC) technical guide
IHC counterstains technical guide

Related Products:

DyLight Reactive Fluors
All Fluorescent Labeling Reagents – including AMCA, FITC, and TRITC
Immunohistochemistry Reagents – including DAPI and Hoechst dyes
Cellular Imaging and Analysis Reagents (formerly Cellomics Products)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
62254 DRAQ5 Fluorescent Probe
Formulation: 5mM solution of proprietary anthraquinone dye
Sufficient For: Approx. 4 x 96 microplate wells
50µL Product Instructions for product #62254 DRAQ5 Fluorescent Probe MSDS for product #62254 DRAQ5 Fluorescent Probe Certificate of Analysis for product #62254 DRAQ5 Fluorescent Probe $195.00

Add to cart
62251 DRAQ5 Fluorescent Probe
Formulation: 5mM solution of proprietary anthraquinone dye
Sufficient For: Approx. 16 x 96 microplate wells
200µL Product Instructions for product #62251 DRAQ5 Fluorescent Probe MSDS for product #62251 DRAQ5 Fluorescent Probe Certificate of Analysis for product #62251 DRAQ5 Fluorescent Probe $350.00

Add to cart
62252 DRAQ5 Fluorescent Probe
Formulation: 5mM solution of proprietary anthraquinone dye
Sufficient For: Approx. 80 x 96 microplate wells
1mL Product Instructions for product #62252 DRAQ5 Fluorescent Probe MSDS for product #62252 DRAQ5 Fluorescent Probe Certificate of Analysis for product #62252 DRAQ5 Fluorescent Probe $1,450.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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