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DTT (Dithiothreitol)


Popular disulfide reducing agent for protein electrophoresis (SDS-PAGE) and other methods.

Pierce DTT (Dithiothreitol)

Thermo Scientific Pierce DTT (Dithiothreitol) is offered as 7.7mg x 48-tube microplates for convenient preparation of 100µL of 0.5M stocks for addition to protein samples for effective reduction of disulfide bonds.

Dithiothreitol is a popular reducing agent for many protein research applications, including reducing polyacrylamide electrophoresis (SDS-PAGE). The No-Weigh Format consists of a 48-microtube tray containing dry, stable, single-use aliquots of the dithiothreitol (DTT). Just puncture the seal of one microtube and add 100µL of water or buffer. In just a few seconds you will have a solution of 500mM DTT, ready for a variety of laboratory methods. Dilute as necessary or add the appropriate volume of the 500mM stock solution to achieve the amount of reducing agent required for your specific application.

Highlights (of the No-Weigh Format):

  • Saves time – just add 100µL water to make 500mM DTT solution in seconds
  • Eliminates waste – makes 100µL at a time, just the right amount for most applications
  • Always fresh – ensures full reducing strength for every use; no need to store stock solutions
Dithiothreitol; DTT

Product Details:

With the convenient No-Weigh Format, a 500mM DTT solution can be made in less than 30 seconds. The unique packaging ensures that the reducing agent is at full strength and able to protect proteins from oxidative damage or reduce any disulfides prior to electrophoresis.

References:

  1. Zahler, W.L. and Cleland, W.W. (1964). Biochemistry 3, 480-482.

Related Resources:

Overview of crosslinking and protein modification
Overview of protein electrophoresis

Related Products:

Bond-Breaker TCEP Solution
All Disulfide Reducing Agents


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20290 DTT (Dithiothreitol)
Formulation: Pure (approx. 98%) white powder of dithiothreitol
Sufficient For: Preparing 64.8mL of 0.5M solution
5g MSDS for product #20290 DTT (Dithiothreitol) Certificate of Analysis for product #20290 DTT (Dithiothreitol) $115.00

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20291 DTT (Dithiothreitol), No-Weigh Format
Formulation: Pure (approx. 98%) white powder of dithiothreitol
Sufficient For: Preparing 100μL of 0.5M DTT per microtube
48 x 7.7mg Product Instructions for product #20291 DTT (Dithiothreitol), No-Weigh Format MSDS for product #20291 DTT (Dithiothreitol), No-Weigh Format Certificate of Analysis for product #20291 DTT (Dithiothreitol), No-Weigh Format $139.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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