Update profile
    Change password
    Sign out

Our content has moved! Check out our protein biology page on lifetechnologies.com  Go now >

DFDNB (1,5-Difluoro-2,4-dinitrobenzene)

For amine-crosslinking across very small distances.

Pierce DFDNB

Thermo Scientific Pierce DFDNB is 1,5-difluoro-2,4-dinitrobenzene, an aryl halide compound with two reactive fluorine atoms that can couple to amine-containing molecules, yielding stable arylamine bonds.


  • Reactive groups: aryl halide (both ends)
  • Reactive towards: amino groups (primary amines)
  • Short distance crosslinks



Product Details:

1,5-Difluoro-2,4-dinitrobenzene; DFDNB
Chemical structure of DFDNB crosslinking reagent.


  1. Chen, X., et al. (1999). Anal. Biochem. 273, 192-203.
  2. Kornblatt, J.A. and Lake, D.F. (1980). Can J. Biochem. 58, 219-224.

Product References:

Crosslinker Application Guide -- search for recent literature references for this product

Related Resources:

Overview of crosslinking
Chemistry of crosslinking

Related Products:

Crosslinkers at a glance (selection guide)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
21525 DFDNB
Formulation: 1,5-Difluoro-2,4-dinitrobenzene; powder solid
Sufficient For: 245mL of typical 1X (1mM) reaction solution, or approx. 25 uses at 2mg reagent per use
50mg Product Instructions for product #21525 DFDNB MSDS for product #21525 DFDNB Certificate of Analysis for product #21525 DFDNB $60.00

Now available on lifetechnologies.com

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Recommend this page

Follow Us
Email Sign-up  Email Announcements

You may also need
22589 - Sulfo-SANPAH

21580 - BS3

33063 - TSAT


Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2015 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747