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Detoxi-Gel Endotoxin Removing Gel


Reduces endotoxin levels in a wide variety of solutions and buffers.

Thermo Scientific Detoxi-Gel Endotoxin Removing Gel and ColumnsThermo Scientific Detoxi-Gel Endotoxin Removing Gel and Columns use Polymixin B ligand immobilized on beaded affinity resin to bind and extract endotoxins from antibody or protein samples.

Detoxi-Gel Endotoxin Removing Gel and quickly remove endotoxins (lipopolysaccharides or LPS) from samples. Even low levels of endotoxins can be toxic to cells or organisms and must be removed before biological samples can be introduced.

Highlights:

  • Resin uses immobilized polymixin B to remove pyrogens by binding their lipid A domains
  • Reduces endotoxin levels in protein solutions, cell culture media, solutions containing pharmacological components and aqueous buffers
  • 1mL of gel removes >9995 EU from a 10,000 EU challenge of LPS (greater than 99% efficiency)
  • 1mL of gel binds 4000-6000 E.U. of the lipopolysaccharide from E. Coli strain 055:BS
  • Protein recovery dependent upon protein type and concentration; some empirical testing required
  • Stable and reusable; resin can be regenerated by stripping off the endotoxins with a 1% deoxycholate solution in pyrogen-poor water; no loss in binding capacity even after 10 regenerations

References:

  1. Kohda, C., et al. (2002). Infect. Immun. 70(3), 1334-1341.
  2. Wang, L., et al. (2002). J. Clin. Invest. 110(8), 1175-1184.
  3. Lei, B., et al. (2002). Infect. Immun. 70(12), 6880-6890.
  4. Kimoto, T., et al. (2003). Infect. Immun. 71(5), 2447-2454.
  5. Madrazo, D.R., et al. (2003). Infect. Immun. 71(9), 5418-5421.
  6. Goh, Y.Y., (2002). Appl. Environ. Microbiol. 68(12), 6343-6352.
  7. Lavelle, E.C., et al. (2003). J. Immunol. 171, 2384-2392.
  8. Ben-Menachem, G., et al. (2003). Proc. Natl. Acad. Sci. USA 100(13), 7913-7918.

Related Resources:

Detoxi-Gel Endotoxin Removing Gel FAQ

Related Products:

Pierce High Capacity Endotoxin Removal Resin
Pierce LAL Chromogenic Endotoxin Quantitation Kit


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20339 Detoxi-Gel Endotoxin Removing Gel
Formulation: Polymixin B on crosslinked 6% beaded agarose slurried in 25% ethyl alcohol
Sufficient For: Binding approx. 10,000 endotoxin units of LPS per mL of resin per use
10mL Product Instructions for product #20339 Detoxi-Gel Endotoxin Removing Gel MSDS for product #20339 Detoxi-Gel Endotoxin Removing Gel Certificate of Analysis for product #20339 Detoxi-Gel Endotoxin Removing Gel $157.00

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20340 Detoxi-Gel Endotoxin Removing Gel
Formulation: Polymixin B on crosslinked 6% beaded agarose slurried in 25% ethyl alcohol
Sufficient For: Binding approx. 10,000 endotoxin units of LPS per mL of resin per use
1000mL Product Instructions for product #20340 Detoxi-Gel Endotoxin Removing Gel MSDS for product #20340 Detoxi-Gel Endotoxin Removing Gel Certificate of Analysis for product #20340 Detoxi-Gel Endotoxin Removing Gel $3,500.00

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20344 Detoxi-Gel Endotoxin Removing Columns, 1 mL
Formulation: 5mL-capacity columns containing 1mL of resin
Sufficient For: Binding approx. 10,000 endotoxin units of LPS per column per use
5 columns Product Instructions for product #20344 Detoxi-Gel Endotoxin Removing Columns, 1 mL MSDS for product #20344 Detoxi-Gel Endotoxin Removing Columns, 1 mL Certificate of Analysis for product #20344 Detoxi-Gel Endotoxin Removing Columns, 1 mL $130.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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