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Immobilized D-Galactose


Purify lectins, galactosidases and other galactose-binding molecules.

Thermo Scientific Pierce D-Galactose AgaroseThermo Scientific Pierce D-Galactose Agarose enables purification of lectins, galactosidases and other galactose-binding molecules.

The immobilized D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This purification method is milder and/or more specific than other methods, allowing protein recovery in a manner that preserves activity for downstream applications.

Highlights:

  • Ligand: thio-alpha-D-galactose
  • Synonyms: thio-alpha-gal, thio-gal
  • Support: crosslinked 6% beaded agarose
  • Binding capacity: at least 20mg jacalin per milliliter of settled resin
  • Format: 50% resin slurry in 0.02% sodium azide
  • Storage: 4°C

Product Details:

Chemical structure of alpha-D-galactose agarose.
 
Chemical structure of Thermo Scientific Pierce D-Galactose Agarose. Each porous agarose bead is 45 to 165µm diameter and contains many billions of thio-alpha-D-galactose groups.

Applications:

  • Human alpha-galactosidase A purification1
  • C-type lectin purification2
  • E. coli heat labile enterotoxin purification3,4
  • Cholera toxin (CT) purification4,5

References:

  1. Yasuda, K., et al. (2004). Efficient and rapid purification of human a-galactosidase A by affinity column chromatography. Protein Expression and Purification. 37: 499-506.
  2. Matsumoto, J. et al. (2001). A novel C-type lectin regulating cell growth, cell adhesion and cell differentiation of the multipotent epithelium in budding tunicates. Development 128:3339-47.
  3. Okamoto, K., et al. (1998) Contribution of the disulfide bond of the A subunit to the action of Escherichia coli heat-labile enterotoxin J. Bacteriol. 180: 1368-74.
  4. Bowman, C.C and Clements, J.D. (2001). Different biological and adjuvant activities of cholera toxin and Escherichia coli heat-labile enterotoxin hybrids. Infec. Immun. 69: 1528-35.
  5. Tinker, J.K., et al. (2003). Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system. Infect. Immun. 71:4093-101.
  6. Hermanson, G.T., et al. (1992) Immobilized Affinity Ligand Techniques. Academic Press Inc.

Related Resources:

Review of affinity purification

Related Products:

Glycoprotein Isolation Kits (ConA and WGA agarose)


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
20372 Pierce D-Galactose Agarose
Formulation: Thio-alpha-D-galactose on 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥ 20mg jacalin per mL resin
5mL Product Instructions for product #20372 Pierce D-Galactose Agarose MSDS for product #20372 Pierce D-Galactose Agarose Certificate of Analysis for product #20372 Pierce D-Galactose Agarose $157.00

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20372B Pierce D-Galactose Agarose
Formulation: Thio-alpha-D-galactose on 6% beaded agarose, slurried in water with sodium azide
Sufficient For: Binding ≥ 20mg jacalin per mL resin
Custom MSDS for product #20372B Pierce D-Galactose Agarose Certificate of Analysis for product #20372B Pierce D-Galactose Agarose

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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