Thermo Scientific Pierce D-Galactose Agarose enables purification of lectins, galactosidases and other galactose-binding molecules.
The immobilized D-galactose is a beaded agarose affinity resin that enables purification of agglutinins, lectins, toxins, and other galactose-binding proteins, N-acetylgalactosamine-binding proteins or carbohydrate-binding proteins. This purification method is milder and/or more specific than other methods, allowing protein recovery in a manner that preserves activity for downstream applications.
- Ligand: thio-alpha-D-galactose
- Synonyms: thio-alpha-gal, thio-gal
- Support: crosslinked 6% beaded agarose
- Binding capacity: at least 20mg jacalin per milliliter of settled resin
- Format: 50% resin slurry in 0.02% sodium azide
- Storage: 4°C
|Chemical structure of Thermo Scientific Pierce D-Galactose Agarose. Each porous agarose bead is 45 to 165µm diameter and contains many billions of thio-alpha-D-galactose groups.
- Human alpha-galactosidase A purification1
- C-type lectin purification2
- E. coli heat labile enterotoxin purification3,4
- Cholera toxin (CT) purification4,5
- Yasuda, K., et al. (2004). Efficient and rapid purification of human a-galactosidase A by affinity column chromatography. Protein Expression and Purification. 37: 499-506.
- Matsumoto, J. et al. (2001). A novel C-type lectin regulating cell growth, cell adhesion and cell differentiation of the multipotent epithelium in budding tunicates. Development 128:3339-47.
- Okamoto, K., et al. (1998) Contribution of the disulfide bond of the A subunit to the action of Escherichia coli heat-labile enterotoxin J. Bacteriol. 180: 1368-74.
- Bowman, C.C and Clements, J.D. (2001). Different biological and adjuvant activities of cholera toxin and Escherichia coli heat-labile enterotoxin hybrids. Infec. Immun. 69: 1528-35.
- Tinker, J.K., et al. (2003). Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system. Infect. Immun. 71:4093-101.
- Hermanson, G.T., et al. (1992) Immobilized Affinity Ligand Techniques. Academic Press Inc.
Review of affinity purification
Glycoprotein Isolation Kits (ConA and WGA agarose)