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Tandem Mass Tag Reagents, Cysteine-Reactive


For protein expression analysis of sulfhydryl components by mass spectrometry.

Cysteine-reactive Thermo Scientific Tandem Mass Tag ReagentsThe cysteine-reactive Thermo Scientific iodoTMT™ (Tandem Mass Tag™) Reagents and Labeling Kit enable measurement of protein and peptide cysteine modifications (S-nitrosylation, oxidation and disulfide bridges) by multiplex quantitative mass spectrometry.

Tandem Mass Tag™ (TMT™) Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. The iodoTMT™ Reagents are sets of isobaric (mass and structure) isomers that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (–SH) groups. Similar to iodoacetamide, iodoTMT Reagents react specifically with reduced cysteines (Cys) in peptides and proteins. IodoTMT Reagents can be differentiated by mass spectrometry (MS), enabling quantitation of the relative abundance of cysteine modifications, such as S-nitrosylation, oxidation and disulfide bonds, in cultured cells grown or treated with different conditions.

Highlights:

  • Specific – only reacts with sulfhydryl groups (reduced, unmodified cysteine residues)
  • Irreversible – labeled proteins and peptides are not susceptible to reducing agents
  • Flexible – options for duplex isotopic (MS) or sixplex isobaric (MS/MS) quantitation
  • Versatile – can be used to study cysteine modifications (e.g. di-sulfide bridges, S-nitrosylation)

Product Details:

IodoTMT Products replace our previously offered CysTMT™ Reagents, which utilized a dithiopyridine reactive group to reversibly label cysteine sulfhydryls. For information about customized reagent sets, please contact our Large Volume and Custom Sales Department.

iodoTMT chemical structures
Thermo Scientific iodoTMT Reagents. (A) iodoTMTzero reagent showing key structural features, including MS/MS fragmentation sites by higher energy collision dissociation (HCD) and electron transfer dissociation (ETD). (B) Structures of iodoTMTsixplex reagents showing the mass (Da) of each reporter and the distribution of normalizing isotopes. Learn about the iodoacetyl reaction scheme.

 

Workflow for tandem mass tag (TMT) analysis of cysteine sulfhydryls with iodoTMTsixplex reagents
Workflow schematic for sixplex reagent experiment. (A) Six different sample conditions can be prepared for labeling with Thermo Scientific iodoTMTsixplex Reagents. Labeled proteins are combined before iodoTMT peptide enrichment using immobilized anti-TMT antibody resin. (B) Subsequent LC-MS/MS analysis of isobaric reporter ions provides quantitation of test samples.

 

More Product Data

References:

  1. Thompson, A., et al. (2003). Tandem mass tags: a novel quantification strategy for comparative analysis of complex protein mixtures by MS/MS. Anal. Chem. 75:1895-204.
  2. Forrester MT, et al. (2009). Detection of protein S-nitroylsation with the biotin technique. Free Radic Biol Med. 46(2):119-126.
  3. Gygi, S.P., et al. (1999). Quantitative analysis of complex protein mixtures using isotopecoded affinity tags. Nat. Biotech. 17:994-9.
  4. Murray C.I., et al. (2012) Identification and quantification of S-nitrosylation by cysteine reactive tandem mass tag switch assay. Mol Cell Proteomics. 11(2): M111.013441.
  5. Yao, C., et al. (2013). Proteomic study of reversible cysteine oxidation in catalase overexpressing mice on a western diet. FASEB J, Apr 2013; 27:810.3.
  6. Pan KT, et al. (2013). Mass spectrometry-based quantitative proteomics for dissecting multiplexed redox cysteine modifications in nitric oxide-protected cardiomyocyte under hypoxia. Antioxidants & Redox Signaling. doi:10.1089/ars.2013.5326.

Related Resources:

Mass Spec Sample Prep Workflow (selection guide)
Mass spectrometry technical guide
Quantitative proteomics technical guide
Service providers that use our mass tagging products
TMT Technology - opens Proteome Sciences website

Related Products:

Immobilized TCEP and other reducing agents
Zeba Spin Desalting Columns
Protease and Phosphatase Inhibitors
TMT Antibody and Enrichment Kit
TMT Amine-reactive Tandem Mass Tag Reagents
SILAC Isotope Labeling Reagents and Kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
90100 iodoTMTzero Label Reagent, 5 x 0.2mg
Formulation: iodoTMT0 label reagent
Sufficient For: Controls in 5 experiments

Kit Contents:
iodoTMT0-126 Label Reagent, 5 x 0.2mg
5-rxn set Product Instructions for product #90100 iodoTMTzero Label Reagent, 5 x 0.2mg MSDS for product #90100 iodoTMTzero Label Reagent, 5 x 0.2mg Certificate of Analysis for product #90100 iodoTMTzero Label Reagent, 5 x 0.2mg $155.00

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90101 iodoTMTsixplex Label Reagent Set, 1 x 0.2mg
Formulation: Set of -126 to -131 iodoTMT6 label reagents
Sufficient For: 1 sixplex (1 x 6-way) experiment

Kit Contents:
iodoTMT6-126 Label Reagent, 0.2mg
iodoTMT6-127 Label Reagent, 0.2mg
iodoTMT6-128 Label Reagent, 0.2mg
iodoTMT6-129 Label Reagent, 0.2mg
iodoTMT6-130 Label Reagent, 0.2mg
iodoTMT6-131 Label Reagent, 0.2mg
6-rxn set Product Instructions for product #90101 iodoTMTsixplex Label Reagent Set, 1 x 0.2mg MSDS for product #90101 iodoTMTsixplex Label Reagent Set, 1 x 0.2mg Certificate of Analysis for product #90101 iodoTMTsixplex Label Reagent Set, 1 x 0.2mg $514.00

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90102 iodoTMTsixplex Label Reagent Set, 5 x 0.2mg
Formulation: Set of -126 to -131 iodoTMT6 label reagents
Sufficient For: 5 sixplex (5 x 6-way) experiments

Kit Contents:
iodoTMT6-126 Label Reagent, 5 x 0.2mg
iodoTMT6-127 Label Reagent, 5 x 0.2mg
iodoTMT6-128 Label Reagent, 5 x 0.2mg
iodoTMT6-129 Label Reagent, 5 x 0.2mg
iodoTMT6-130 Label Reagent, 5 x 0.2mg
iodoTMT6-131 Label Reagent, 5 x 0.2mg
30-rxn set Product Instructions for product #90102 iodoTMTsixplex Label Reagent Set, 5 x 0.2mg MSDS for product #90102 iodoTMTsixplex Label Reagent Set, 5 x 0.2mg Certificate of Analysis for product #90102 iodoTMTsixplex Label Reagent Set, 5 x 0.2mg $1,860.00

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90103 iodoTMTsixplex Isobaric Mass Tag Labeling Kit
Formulation: Multi-component kit with -126 to -131 iodoTMT6 label reagents
Sufficient For: 5 complete sixplex (5 x 6-way) experiments

Kit Contents:
iodoTMT6-126 Label Reagent, 5 x 0.2mg
iodoTMT6-127 Label Reagent, 5 x 0.2mg
iodoTMT6-128 Label Reagent, 5 x 0.2mg
iodoTMT6-129 Label Reagent, 5 x 0.2mg
iodoTMT6-130 Label Reagent, 5 x 0.2mg
iodoTMT6-131 Label Reagent, 5 x 0.2mg
HES Buffer, 15mL
Bond-Breaker TCEP Solution, 0.5mL
DTT, No-Weigh Format, 8 x 7.7mg
Trypsin, 5 x 20μg
Trypsin Storage Solution, 250μL
Albumin (bovine), 2.5mg
30-rxn kit Product Instructions for product #90103 iodoTMTsixplex Isobaric Mass Tag Labeling Kit MSDS for product #90103 iodoTMTsixplex Isobaric Mass Tag Labeling Kit Certificate of Analysis for product #90103 iodoTMTsixplex Isobaric Mass Tag Labeling Kit $2,050.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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