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Cypridina Luciferase Reporter Assay Vectors


Cypridina reporter plasmids to clone genes for luciferase assays.

Thermo Scientific pMCS-Cypridina Luciferase Vector

The Thermo Scientific pMCS-Cypridina Luc Vector is a multiple cloning site plasmid designed to accept a promoter sequence for study of gene regulation using the naturally secreting Cypridina luciferase reporter.

The Cypridina Luc Vectors contain a gene cloned from the marine ostracod, Cypridina noctiluca. The gene encodes a naturally-secreted bioluminescent Cypridina luciferase (62kDa), which enables measurement of the reporter activity in media (for real-time assays) and in cell lysates. The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential. The pCMV and pTK vectors have the luciferase gene under the CMV (Cytomegalovirus) promoter and Herpes Simplex Virus (HSV) thymidine kinase (TK) promoter, respectively. These constitutive expression vectors can be used as normalization controls to account for experimental variation in combination with other reporters.

Highlights:

  • Naturally-secreting Cypridina luciferase gene, optimized for high expression in mammalian systems
  • Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
  • Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
  • Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
  • High-copy pUC bacterial DNA replication origin
  • Two control vectors available with strong (CMV) or weak (TK) constitutive promoters for co-transfection and normalization

Product Details:

The pMCS-, pCMV- and pTK-Cypridina Luc Vectors encode the Cypridina luciferase reporter with excellent light intensity.

Map of pMCS-Cypridina Luc Vector for luciferase assays
Plasmid map of the Thermo Scientific pMCS-Cypridina Luc Vector. See the following downloadable files for complete vector and cloning site sequences.

 

Download the complete sequence (.txt) and printable data sheet (.pdf) of each vector.
Vector Vector Information Files
pMCS-Cypridina Luc pMCS-Cypridina Luc Sequence (.txt) Data Sheet (.pdf)
pCMV-Cypridina Luc pCMV-Cypridina Luc Sequence (.txt) Data Sheet (.pdf)
pTK-Cypridina Luc pTK-Cypridina Luc Sequence (.txt) Data Sheet (.pdf)

These vectors are subject to a limited use label license.

More Product Data

General References:

  1. Nakajima, Y., et al. (2004). cDNA Cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca. Biosci. Biotechnol. Biochem. 68(3):565-570.

Product References:

  1. Varsano, T., et al. (2013). Inhibition of melanoma growth by small molecules that promote the mitochondrial localization of ATF2. Clin. Cancer Res. 19:2710-22.

Related Resources:

Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide

Related Products:

Cypridina Flash, Glow and Dual Luciferase Assay Kits
All Pierce Luciferase Assay Kits and Vectors
Custom Reporter Vector Cloning Service


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
16149 pMCS-Cypridina Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16149 pMCS-Cypridina Luc Certificate of Analysis for product #16149 pMCS-Cypridina Luc $57.00

Add to cart
16150 pCMV-Cypridina Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16150 pCMV-Cypridina Luc Certificate of Analysis for product #16150 pCMV-Cypridina Luc $57.00

Add to cart
16151 pTK-Cypridina Luc
Formulation: DNA at 0.5µg/µL in 10mM Tris-HCl, pH 8.5
10µg MSDS for product #16151 pTK-Cypridina Luc Certificate of Analysis for product #16151 pTK-Cypridina Luc $57.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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