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Cyclic GMP Competitive ELISA (Lysates)


Microplate competitive sandwich ELISA kit for cyclic GMP (cGMP).

Thermo Scientific Cyclic GMP (cGMP) ELISA Kits

Assay cyclic GMP (cGMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic GMP Competitive ELISA Kit.

This cyclic GMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic guanosine monophosphate (cGMP) in samples treated with 0.1M HCl. The assay is based on the competition between cGMP in the standard or sample and alkaline phosphatase conjugated cGMP (cGMP-AP) for a limited amount of cGMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cGMP in the sample increases, the amount of cGMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Highlights:

  • Target: cyclic guanosine monophosphate (cyclic GMP, cGMP)
  • Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
  • Measurement: absorbance at 405nm minus absorbance at 570nm
  • Assay Range: 0.8 to 500pmol/mL (non-acetylated); 0.08 to 50pmol/mL (acetylated)
  • Sensitivity: 0.6pmol/mL (non-acetylated); 0.06pmol/mL (acetylated)
  • Precision: dependent upon sample type, level and assay (see instructions)
  • Sample Types: cell culture supernatant, cell lysate, tissue homogenate
  • Total Assay Time: 3 hours (non-acetylated); 2 hours (acetylated)
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with GMP, GTP, cAMP, AMP, ATP, cUMP, or CTP

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, treatment reagents, buffers, standard, substrate, and stop solution.

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMSCGMPL Cyclic GMP Competitive ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Rabbit IgG Precoated 96-well Plate, 1 plate
cGMP Antibody, 5mL
cGMP-AP Conjugate, 5mL
0.1M HCl, 30mL
Neutralizing Reagent, 6mL
Triethylamine, 2mL
Acetic Anhydride, 1mL
cGMP Standard, 0.5mL
10X Wash Buffer, 30mL
p-NPP Substrate Solution, 20mL
Stop Solution, 6mL
Plate Sealer, 1 each
1-plate kit MSDS for product #EMSCGMPL Cyclic GMP Competitive ELISA Kit $399.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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