Update profile
    Change password
    Sign out

Cyclic AMP Competitive ELISA


Microplate competitive sandwich ELISA kit for cyclic AMP (cAMP).

Thermo Scientific Cyclic AMP (cAMP) ELISA Kits

Assay cyclic AMP (cAMP) levels in culture supernatant, cell lysate and tissue homogenate samples (treated with 0.1M HCl and acetylated or left non-acetylated) using our Thermo Scientific Cyclic AMP Competitive ELISA Kit.

This cyclic AMP competitive ELISA kit is an immunoassay for the quantitative determination of cyclic adenosine monophosphate (cAMP) in samples treated with 0.1M HCl. The assay is based on the competition between cAMP in the standard or sample and alkaline phosphatase conjugated cAMP (cAMP-AP) for a limited amount of cAMP monoclonal antibody bound to an anti-rabbit IgG precoated 96-well plate. As the concentration of cAMP in the sample increases, the amount of cAMP-AP captured by the coating antibody decreases. Thus, there is an inverse relationship between optical density (OD) and the amount of analyte in the sample.

Highlights:

  • Target: cyclic adenosine monophosphate (cyclic AMP, cAMP)
  • Format: colorimetric ELISA (p-NPP substrate) in clear 96-well plate
  • Measurement: absorbance at 405nm minus absorbance at 570nm
  • Assay Range: 0.78 to 200pmol/mL (non-acetylated); 0.08 to 20pmol/mL (acetylated)
  • Sensitivity: 0.39pmol/mL (non-acetylated); 0.04pmol/mL (acetylated)
  • Precision: dependent upon sample type, level and assay (see instructions)
  • Sample Types: cell culture supernatant, cell lysate, tissue homogenate
  • Total Assay Time: 3 hours (non-acetylated); 2 hours (acetylated)
  • Sample Size: 100μL
  • Specificity: no significant cross-reaction with AMP, ATP, cGMP, GMP, GTP, cUMP, or CTP

Includes:

Complete kit contains pre-coated antibody-plate, detection antibodies, treatment reagents, buffers, standard, substrate, and stop solution.

References:

Related Resources:

Related Products:

Notice:

This product is for In Vitro experimental use only. Not for resale without express authorization.


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
EMSCAMPL Cyclic AMP Competitive ELISA Kit
Formulation: Complete ELISA kit with coated 96-well plate
Sufficient For: 96 microplate wells

Kit Contents:
Anti-Rabbit Precoated 96-well IgG Plate, 1 each
cAMP Antibody, 5mL
cAMP-AP Conjugate, 5mL
0.1M HCl, 30mL
Neutralizing Reagent, 6mL
Triethylamine, 2mL
Acetic Anhydride, 1mL
cAMP Standard, 0.5mL
10X Wash Buffer, 30mL
p-NPP Substrate Solution, 20mL
Stop Solution, 6mL
Plate Sealer, 1 each
1-plate kit MSDS for product #EMSCAMPL Cyclic AMP Competitive ELISA Kit $399.00

Add to cart

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

Share This Page
 
 

Follow Us
  Email Sign-up  Email Announcements

 



Antibodies  |   Molecular Biology   |   Cell Biology   |  Thermo Scientific  |   * Trademarks  |   Privacy Statement
© 2014 Thermo Fisher Scientific Inc.   |   3747 N Meridian Rd, Rockford, IL USA 61101   |   1-800-874-3723  or  815-968-0747