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Pierce Copper Coated Plates, High Binding Capacity


Boost plate-based assay performance by binding more His-tagged protein.

Pierce Copper Coated High Binding Capacity (HBC) Plates

Thermo Scientific Pierce Copper Coated High Binding Capacity (HBC) Plates use an exclusive coating process to increase the amount of histidine-tagged protein that will bind to the plate surface.

These copper coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metals such as copper. Pierce Copper Coated Plates have high binding capacity because they are coating using an exclusive process that increases the amount of histidine-tagged protein that will bind to the plate surface. Although copper is less discriminating in its binding specificity than Nickel, the higher binding capacity of these plates is ideal for high throughput screening applications that need improved sensitivity and greater dynamic range. The copper-coated plates are available as clear, white or black plates that can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.

Highlights:

  • Quantitate previously undetectable histidine-tagged proteins
  • Wider dynamic range with four-fold greater capacity than regular nickel chelate-coated plate
  • Copper chelate provides greater binding capacity than nickel
  • The detection limit is 0.1 pg of polyhistidine tagged fusion protein per well
  • Pre-blocked for the ultimate convenience

Product Details:

Binding performance of nickel vs. copper coated plates. Comparison of Thermo Scientific Pierce Nickel Coated Plates to Pierce Copper Coated High Binding Capacity (HBC) Plates. Performance was evaluated comparing the amount of fluorescent signal produced by captured His-tagged fluorescent protein. Binding comparison of Nickel and Copper Coated  Microplates.

Note: Copper coated plates are for use with purified His-tagged proteins. Typical cell lysates contain non-his-tagged proteins that may bind to copper, which can reduce binding capacity and signal-to-noise ratios. For cell lysates, use Thermo Scientific Pierce Nickel Coated Plates.

Related Resources:

Assay development and ELISA technical guide
His-tagged proteins technical guide

Related Products:

Nickel Coated Plates
Resins for His-tagged Protein Purification


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
15143 Copper Coated High Capacity Plates, Clear, 96-Well
Formulation: Clear polystyrene 96-well plates coated and blocked at 200μL/well with proprietary copper-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 35pmol His-tagged protein per well
5 plates Product Instructions for product #15143 Copper Coated High Capacity Plates, Clear, 96-Well MSDS for product #15143 Copper Coated High Capacity Plates, Clear, 96-Well Certificate of Analysis for product #15143 Copper Coated High Capacity Plates, Clear, 96-Well $229.00

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15146 Copper Coated High Capacity Plates, Clear, 8-Well Strip
Formulation: Clear polystyrene 96-well strip plates coated and blocked at 200μL/well with proprietary copper-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 35pmol His-tagged protein per well
5 plates Product Instructions for product #15146 Copper Coated High Capacity Plates, Clear, 8-Well Strip MSDS for product #15146 Copper Coated High Capacity Plates, Clear, 8-Well Strip Certificate of Analysis for product #15146 Copper Coated High Capacity Plates, Clear, 8-Well Strip $246.00

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15147 Copper Coated High Capacity Plates, White, 96-Well
Formulation: White polystyrene 96-well plates coated and blocked at 200μL/well with proprietary copper-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 35pmol His-tagged protein per well
5 plates Product Instructions for product #15147 Copper Coated High Capacity Plates, White, 96-Well MSDS for product #15147 Copper Coated High Capacity Plates, White, 96-Well Certificate of Analysis for product #15147 Copper Coated High Capacity Plates, White, 96-Well $246.00

Add to cart
15148 Copper Coated High Capacity Plates, Black, 96-Well
Formulation: Black polystyrene 96-well plates coated and blocked at 200μL/well with proprietary copper-presenting molecular solution containing bovine serum albumin.
Sufficient For: Binding approx. 35pmol His-tagged protein per well
5 plates Product Instructions for product #15148 Copper Coated High Capacity Plates, Black, 96-Well MSDS for product #15148 Copper Coated High Capacity Plates, Black, 96-Well Certificate of Analysis for product #15148 Copper Coated High Capacity Plates, Black, 96-Well $246.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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