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Controlled Protein-Protein Crosslinking Kit


Complete set of reagents for two-step protein conjugation.

Pierce Controlled Protein-Protein Crosslinking Kit

The Thermo Scientific Pierce Controlled Protein-Protein Crosslinking Kit contains essential crosslinkers, modification reagents and buffers for conjugating antibodies, enzymes and other purified proteins needed as probes.

This crosslinking kit contains everything you need to help you insert an unbreakable molecular bridge between practically any two purified proteins and do it successfully right out of the box.

Highlights:

  • Easy-to-control, stepwise coupling of two proteins — You are in charge of the chemistry. Stop and restart at convenient points or go to completion. It is your choice.
  • Better protocol…logically presented and clearly worded instructions which will be appreciated by novice and expert — Instructions teach the method and chemistry of this crosslinking strategy to the user while providing a high likelihood of success. Great for life scientists attempting to prepare a conjugate or crosslink two proteins for the first time.
  • Reliable non-cleavable heterobifunctional crosslinking agent — A very popular choice for a crosslinking agent was selected for the kit offering proven chemistry ,highly stabile intermediates and efficient formation of the target conjugate adding to the ease of use and likelihood of success.
  • All reagents necessary to affect the crosslinking — Cuts costs over purchasing “catalog size” packaging of all the components by more than 60%. If it's not in the kit, you don’t need it!
  • Includes disulfide (S-S) reductants and thiolation reagents — Allows for all possibilities regarding the state of the –SH group on one of the proteins to be coupled.
  • Ellman’s Reagent colorimetric –SH group determination chemistry built into the protocols —You have the option to see how well your reactions went. eg. determine the extent of –SH introduction or estimate the incorporation of maleimide reactive group.

References:

  1. Hermanson, G.T. (1996). Bioconjugate Techniques. Academic Press: London.
  2. Riddle, P.W., et al. (1983). Methods Enzymol. 91(8), 49-60.
  3. Duncan, R.J.S., et al. (1983). Anal. Biochem. 132, 68-73.
  4. Jocelyn, P.C. (1972). Biochemistry of the SH Group. Academic Press: London, pp. 40-42.
  5. Imagawa, M., et al. (1982). J. Appl. Biochem. 4, 41-57.
  6. Hashida, S., et al. (1984). J. Appl. Biochem. 6, 56-63.

Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
23456 Controlled Protein-Protein Crosslinking Kit
Formulation: Multi-component kit with Sulfo-SMCC and SATA reagents
Sufficient For: One conjugation with two purified proteins (up to 5mg each)

Kit Contents:
Sulfo-SMCC Crosslinking Agent, 2mg
Activation/Conjugation Buffer (10X), 20mL
PBS packs (each makes 500mL), 2 packs
2-Mercaptoethylamine-HCl, 6mg
Immobilized-Reductant, 0.2mL
SATA, 2mg
Dimethylformamide (DMF), 1mL
Hydroxylamine-HCl, 5mg
Dextran Desalting Column, 2 x 10mL
Column Extender, 2 ea.
Cysteine-HCl, 20mg
Ellman's Reagent, 2mg
1-rxn kit Product Instructions for product #23456 Controlled Protein-Protein Crosslinking Kit MSDS for product #23456 Controlled Protein-Protein Crosslinking Kit Certificate of Analysis for product #23456 Controlled Protein-Protein Crosslinking Kit $388.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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