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Pierce CN/DAB Substrate Kit

TThe best of chloronapthol and diaminobenzidine in one kit.

Pierce CN/DAB Substrate Kit

Thermo Scientific Pierce CN/DAB Substrate Kit includes substrate and peroxide solutions for combined chloronaphthol- and diaminobenzidine-based detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

This kit combines two popular chromogenic peroxidase substrate compounds (4-CN and DAB) in a single, stable, 10X stock solution. This CN/DAB solution produces an intense dark black precipitate at sites of bound HRP-conjugated antibodies on probed blots and tissue samples. The Pierce CN/DAB Substrate Kit can be used in Western immunoblotting techniques as well as in dot blotting assays. The kit includes the 10X solution of CN/DAB and accompanying 1X Stable Peroxide Substrate Buffer.


  • HRP substrate – for detection of horseradish peroxidase activity on solid media via the combined action of CN (4-chloro-1-naphthol) and DAB (3,3'-diaminobenzidine tetrahydrochloride)
  • Chromogenic – no special equipment needed for visualization; yields intense black bands that are easy to photograph
  • Easy-to-use kit – substrate stock solution and stable peroxide substrate buffer mean that there's no dry powders to measure and dissolve
  • Stable – store refrigerated at 4°C for at least one year


  • CN/DAB Solution (10X) and Stable Peroxide Substrate Solution (1X)

General Reference:

  1. Young, P.R. (1989). J. Virol. Methods 24, 227-235.

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
34000 CN/DAB Substrate Kit
Formulation: Proprietary organic-solvent and aqueous-buffer solutions
Sufficient For: 25 uses at 10mL each

Kit Contents:
CN/DAB (10X), 25mL
Stable Peroxide Buffer, 250mL
275mL kit Product Instructions for product #34000 CN/DAB Substrate Kit MSDS for product #34000 CN/DAB Substrate Kit Certificate of Analysis for product #34000 CN/DAB Substrate Kit $116.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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