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Pierce Chloronaphthol Substrates

A precipitating blue-purple HRP substrate for Western blot and IHC.

Pierce Chloronaphthol (4-CN) Substrates

Thermo Scientific Pierce Chloronaphthol (4-CN) Substrates are powders, tablets and ready-made solutions of 4-chloro-1-naphthol for chromogenic detection of horseradish peroxidase (HRP) activity in Western blot and tissue staining methods.

Chloronaphthol (4-CN, MW = 178.6) reacts with HRP to form a distinct blue to blue-purple product to localize and visualize the activity of peroxidase-conjugated antibodies on blots or fixed tissue samples. The HRP substrate is not as sensitive or stable as other chromogenic substrates, such as TMB and DAB. However, the blue-purple precipitate photographs well, and its alcohol-solubility is a property that can be utilized in certain double-staining applications.


  • HRP substrate – for detection of horseradish peroxidase activity on solid media
  • Chromogenic – no special equipment needed for visualization
  • Blue-purple – easy to photograph in color or black-and-white
  • Package options – choose powder, tablets, or ready-to-use solution

Highlights of Pierce CN Solution (Part No. 34012):

  • Ready-to-use – no ethanol or methanol required to dissolve; no dilution necessary for use
  • Complete – does not require buffer or addition of hydrogen peroxide (Part No. 34062)
  • Pre-filtered – does not need to be filtered before use like powders or tablets after they are dissolved
  • Stable – store refrigerated at 4°C for at least one year

Related Products:

Pierce Stable Peroxide Buffer (10X)

Ordering Information
Product # Description Pkg. Size Instructions MSDS CofA Price
34010 Chloronaphthol Powder
Formulation: 4-Chloro-1-naphthol (4-CN) powder (≥ 99% pure)
Sufficient For: 830 uses at 1X (3mg/mL) and 10mL each
25g Product Instructions for product #34010 Chloronaphthol Powder MSDS for product #34010 Chloronaphthol Powder Certificate of Analysis for product #34010 Chloronaphthol Powder $74.00

Now available on lifetechnologies.com
34012 1-Step Chloronaphthol Solution
Formulation: 4-Chloro-1-naphthol (4-CN) in proprietary buffer containing peroxide
Sufficient For: 25 uses at 10mL each
250mL Product Instructions for product #34012 1-Step Chloronaphthol Solution MSDS for product #34012 1-Step Chloronaphthol Solution Certificate of Analysis for product #34012 1-Step Chloronaphthol Solution $85.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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