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Chemiluminescent Nucleic Acid Detection Module


The alternative to radioisotopic detection with Northern or Southern blots.

Pierce Chemiluminescent Nucleic Acid Detection Module

The Thermo Scientific Pierce Chemiluminescent Nucleic Acid Detection Module is a complete system for detection of biotin-labeled nucleic acid probes in various membrane blotting applications.

Nearly any DNA or RNA method based on nitrocellulose or nylon membranes and biotinylated probes can be detected with sensitivity equivalent to traditional radioactive (32P) systems. The module consists of the essential detection components used in the Thermo Scientific North2South Kit and Thermo Scientific LightShift EMSA Kit. The system includes an enhanced luminol substrate for horseradish peroxidase (HRP) with optimized blocking and wash buffers that provide for fast, trouble-free detection on film or by CCD camera. Obtain publication-quality blots having excellent signal and low background.

Highlights:

  • Fast and sensitive – achieve the same speed and detection levels you’ve come to expect with Thermo Scientific North2South and LightShift Kits
  • Versatile – kit protocol (included) is applicable to any biotin-based nucleic acid detection system: Northerns, Southerns, EMSA, RPAs and colony lifts
  • Flexible – kit contains only the critical detection components for those who already have biotin-labeled probes and an optimized hybridization method developed

Product Details:

Chemiluminescent Northern blotting
Northern blot analysis of mouse total RNA using the Thermo Scientific Chemiluminescent Nucleic Acid Detection Module. Total RNA samples (1µg) prepared from the indicated tissues were electrophoresed on a denaturing 1% agarose gel, transferred to Biodyne B Positive Nylon, UV crosslinked and hybridized with a biotinylated c-myc/exon 3 RNA probe (4ng/mL). Exposure time to film was 1 minute.

References:

  1. Matata, B. M. and Galiñanes, M. (2002). J. Biol Chem. 277(3), 2330–2335.
  2. Oltra, E., et al. (2003). Endocrinology 144(7), 3148-3158.

Related Products:

Biodyne B Nylon Membrane
Other Products for Nucleic Acid Detection


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89880 Chemiluminescent Nucleic Acid Detection Module Kit
Formulation: Multi-component kit with reagents for detection of nucleic acid blots based on biotinylated probes
Sufficient For: 800cm2 of membrane (10 mini-gel blots)

Kit Contents:
Stabilized Streptavidin-HRP, 1.5mL
Luminol/Enhancer Solution, 80mL
Stable Peroxide Solution, 80mL
Blocking Buffer (Part No. 89880A), 500mL
Wash Buffer (4X), 500mL
Substrate Equilibration Buffer, 500mL
10-blot kit Product Instructions for product #89880 Chemiluminescent Nucleic Acid Detection Module Kit MSDS for product #89880 Chemiluminescent Nucleic Acid Detection Module Kit Certificate of Analysis for product #89880 Chemiluminescent Nucleic Acid Detection Module Kit $369.00

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89880A Nucleic Acid Detection Blocking Buffer
Formulation: Proprietary aqueous buffered solution, pH 7.5
Sufficient For: 800cm2 of membrane (10 mini-gel blots)
500mL MSDS for product #89880A Nucleic Acid Detection Blocking Buffer $174.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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