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CHAPS Detergent


Zwitterionic detergent that protects the native state of proteins.

CHAPS

Thermo Scientific CHAPS is a zwitterionic detergent that is especially well suited for protecting the native state of proteins.

CHAPS is sulfobetaine derivative of cholic acid. This zwitterionic detergent is useful for membrane protein solubilization when it is important to maintain protein activity. CHAPS detergent is soluble over a wide range of pH (2 to 12) and is easily removed from solution by dialysis because it has a high critical micelle concentration (CMC).

Highlights:

  • Versatile zwitterionic detergent
  • Provides mild but effective lysis of cultured mammalian cells
  • Nondenaturing and generally does not inactivate protein functions
  • Can be removed from solution by dialysis
  • Commonly used for isoelectric focusing (IEF) and 2D electrophoresis

Applications:

  • Solubilizing the opiate receptor from rat brain cell membranes, while retaining the reversible opiate binding property of the receptor 1
  • Purifying microsomal signal peptidase as a complex 2
  • Solubilizing dopamine D 2,3, muscarinic 4, and opiate receptors 5 from brain tissue

Product Details:

CHAPS has been successfully used to solubilize intrinsic membrane proteins and receptors and maintain the functional capability of the protein of interest. CHAPS has also been used in combination with nonionic detergents such as NP-40 for nondenaturing gel electrophoresis applications. CHAPS has largely replaced NP-40 in isoelectric focusing (IEF) applications, where it prevents streaking in certain pH ranges. The neutral charge of CHAPS and its disaggregating properties are believed to be responsible for the improvement.

Chemical structure of CHAPS detergent
Chemical structure of CHAPS detergent.

Properties of CHAPS Detergent:

  • Chemical Name: 3-[(3-Cholamidopropyl) dimethylammonio]-1-propanesulfonate
  • Molecular Weight: 614.88g
  • Detergent Class: Zwitterionic
  • Aggregation Number: 10
  • Micelle Molecular Weight: 6149g
  • Critical Micelle Concentration (CMC): 8 to 10mM (0.4920 to 0.6150%, w/v)
  • Cloud Point: ≥100°C
  • Dialyzable: Yes

Specifications for CHAPS:

  • Visual: White free-flowing powder, free of foreign matter.
  • Solubility: A 10% (aq) solution is clear, colorless.
  • Identity: The I.R. scan must show only peaks characteristic for the structure and functional groups of the compound being tested.
  • Purity: (TLC) One major spot. A small, secondary spot is acceptable.

References:

  1. Simonds, W.F., et al. (1980). Proc. Natl. Acad. Sci. (USA) 77, 4623-4627.
  2. Evans, E.A., et al. (1986). Proc. Natl. Acad. Sci. (USA) 83, 581-585.
  3. Kuno, T., et al. (1984). J. Neurochem. 41, 841.
  4. Kuno, T., et al. (1983). BBRC 112, 948.
  5. Chow, T., et al. (1983). Mol. Pharmacol. 24, 203.

Related Resources:

Review of detergents for cell lysis

Related Products:

Detergent Sampler Pack
Other Detergents for Protein Methods
Detergent Removal Resins


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
28300 CHAPS
Formulation: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
5g MSDS for product #28300 CHAPS Certificate of Analysis for product #28300 CHAPS $126.00

Add to cart
28299 CHAPS
Formulation: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
100g MSDS for product #28299 CHAPS Certificate of Analysis for product #28299 CHAPS $1,028.00

Add to cart
28299B CHAPS
Formulation: 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate
Custom MSDS for product #28299B CHAPS

Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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