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Pierce Centrifuge Columns


Columns to handle a wide variety of resin volumes for affinity purification.

Pierce Centrifuge Columns

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40µL to 10mL of an affinity support for purification.

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results!

Highlights:

Specifications of Pierce Centrifuge Columns. All columns are made from low protein binding polypropylene for compatibility with protein purification, and fit into standard centrifuge tubes for use in any centrifuge.
Column Model Details

0.8mL Pierce Centrifuge Column
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0.8mL Centrifuge Columns

Total volume: 800µL
Resin volume: 40-400µL
Filter type, pore size: Polyethylene filter, ~30µm pore size
Receiver tube: Fits standard microcentrifuge tubes
Caps: O-ring screw-top cap
Twist-off bottom

2mL Pierce Centrifuge Column
View Dimensions of
2mL Centrifuge Columns

Total volume: 5mL (2mL resin bed, 3mL reservoir)
Resin volume: 2mL
Filter type, pore size: Polyethylene filter, ~30µm pore size
Receiver tube: Fits standard 15mL conical centrifuge tubes
Caps: Screw-top cap
Twist-off bottom and press-on cap to reseal

5mL Pierce Centrifuge Column
View Dimensions of
5mL Centrifuge Columns

Total volume: 8mL (5mL resin bed, 3mL reservoir)
Resin volume: 5mL
Filter type, pore size: Polyethylene filter, ~30µm pore size
Receiver tube: Fits standard 15mL conical centrifuge tubes
Caps: Screw-top cap
Twist-off bottom and press-on cap to reseal

10mL Pierce Centrifuge Column
View Dimensions of
10mL Centrifuge Columns

Total volume: 22mL (10mL resin bed, 12mL reservoir)
Resin volume: 10mL
Filter type, pore size: Polyethylene filter, ~30µm pore size
Receiver tube: Fits standard 50mL conical centrifuge tubes
Caps: Screw-top cap
Twist-off bottom and press-on cap to reseal

Column Extender for Pierce Centrifuge Columns
View Dimensions of
Column Extenders

Increases column reservoir volume by 35 to 40mL
Designed especially for use in gravity-flow procedures
Fits 2mL, 5mL and 10mL Pierce Centrifuge Columns
Made of polypropylene

Applications:

  • Affinity purification, affinity chromatography
  • Immunodepletion
  • Ion exchange resins
  • Gel filtration, desalting, buffer exchange

Related Resources:

Review of affinity purification methods
Review of desalting and buffer-exchange methods

Related Products:

Microcentrifuge spin cups and columns
Affinity chromatography supports
Immunoprecipitation kits


Ordering Information
 
Product # Description Pkg. Size Instructions MSDS CofA Price
89868 Pierce Centrifuge Columns, 0.8mL
Formulation: Polypropylene (800µL capacity); Polyethylene filter (30µm pore size); Screw-top caps
Sufficient For: Microcentrifuge procedures with 40 to 400µL resin beds
50-columns Product Instructions for product #89868 Pierce Centrifuge Columns, 0.8mL Certificate of Analysis for product #89868 Pierce Centrifuge Columns, 0.8mL $67.00

Add to cart
89869 Pierce Centrifuge Columns, 0.8mL
Formulation: Polypropylene (800µL capacity); Polyethylene filter (30µm pore size); Screw-top caps
Sufficient For: Microcentrifuge procedures with 40 to 400µL resin beds
4 x 50 columns Product Instructions for product #89869 Pierce Centrifuge Columns, 0.8mL $207.00

Add to cart
89869B Pierce Centrifuge Columns, 0.8mL
Formulation: Polypropylene (800µL capacity); Polyethylene filter (30µm pore size); Screw-top caps
Sufficient For: Microcentrifuge procedures with 40 to 400µL resin beds
Custom Certificate of Analysis for product #89869B Pierce Centrifuge Columns, 0.8mL
89896 Pierce Centrifuge Columns, 2mL
Formulation: Polypropylene (5mL capacity); Polyethylene filter (30µm pore size); Screw-top and press-on bottom caps
Sufficient For: Centrifuge procedures with 2mL resin beds and 15mL conical centrifuge tubes for collection
25 columns Product Instructions for product #89896 Pierce Centrifuge Columns, 2mL $42.00

Add to cart
89897 Pierce Centrifuge Columns, 5mL
Formulation: Polypropylene (8mL capacity); Polyethylene filter (30µm pore size); Screw-top and press-on bottom caps
Sufficient For: Centrifuge procedures with 5mL resin beds and 15mL conical centrifuge tubes for collection
25 columns Product Instructions for product #89897 Pierce Centrifuge Columns, 5mL $47.00

Add to cart
89898 Pierce Centrifuge Columns, 10mL
Formulation: Polypropylene (22mL capacity); Polyethylene filter (30µm pore size); Screw-top and press-on bottom caps
Sufficient For: Centrifuge procedures with 10mL resin beds and 50mL conical centrifuge tubes for collection
25 columns Product Instructions for product #89898 Pierce Centrifuge Columns, 10mL $54.00

Add to cart
89898B Pierce Centrifuge Columns, 10mL
Formulation: Polypropylene (22mL capacity); Polyethylene filter (30┬Ám pore size); Screw-top and press-on bottom caps
Custom
69707 Pierce Column Extenders
Formulation: Polypropylene (35mL capacity) to fit Part No. 89896, 89897, 89898
Sufficient For: Increasing column reservoir volumes by approx. 35mL
10 extenders $30.00

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Instructions | MSDS | CofA
Product Instructions | MSDS | CofA  

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