The Thermo Scientific Pierce Luciferase Cell Lysis Buffer effectively lyses adherent cells or pelleted suspension cells for analysis of promoter expression in luciferase assays.
Pierce Luciferase Cell Lysis Buffer is effective for preparing high-yield mammalian cell lysates from either adherent cells or pelleted suspension cells for analysis in luciferase reporter assays. The reagent is compatible with all Thermo Scientific Pierce Luciferase Assay Kits. The cell lysis buffer has been validated for use with COS-7, NIH3T3, Jurkat and other cell types. Lysates are produced rapidly and easily for luciferase assays and are also compatible with BCA and Coomassie (Bradford) protein assays.
- Compatible with all Pierce Luciferase Reporter Assays
- Enables rapid and efficient lysis
- Tested compatibility with multiple Mammalian cell lines
- Compatible with protein assays (e.g., Coomassie Plus and BCA)
Pierce Luciferase Cell Lysis Buffer has been tested and validated to produce luciferase assay-compatible lysates from a variety of cell types. Essentially complete lysis of adherent cells has been observed with nearly all of the cell types tested to date, including COS-7, NIH3T3, Hepa 1-6, 293, CHO, MDA, MB 231, Jurkat and FM2 cells. Pierce Luciferase Cell Lysis Buffer provides rapid and efficient cell lysis and is compatible with protein assays, such as the Thermo Scientific Coomassie Plus (Bradford) Assay (Part No. 23200) and the Thermo Scientific Pierce BCA Protein Assay (Part No. 23227).
Review of Luciferase Reporters
Luciferase Reporter Assay Selection Guide
Pierce Luciferase Assay Kits and Vectors
Other Cell Lysis Reagents
Protein Assay Reagents and Kits